Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures
Por favor, use este identificador para citar o enlazar este ítem:
http://hdl.handle.net/10045/67477
Registro completo de metadatos
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor | Proteómica y Genómica Funcional de Plantas | es_ES |
dc.contributor.author | Hidalgo, Diego | - |
dc.contributor.author | Abdoli-Nasab, Maryam | - |
dc.contributor.author | Jalali-Javaran, Mokhtar | - |
dc.contributor.author | Bru-Martinez, Roque | - |
dc.contributor.author | Cusidó, Rosa M. | - |
dc.contributor.author | Corchete, Purificación | - |
dc.contributor.author | Palazon, Javier | - |
dc.contributor.other | Universidad de Alicante. Departamento de Agroquímica y Bioquímica | es_ES |
dc.date.accessioned | 2017-06-21T10:43:51Z | - |
dc.date.available | 2017-06-21T10:43:51Z | - |
dc.date.issued | 2017-09 | - |
dc.identifier.citation | Plant Physiology and Biochemistry. 2017, 118: 130-137. doi:10.1016/j.plaphy.2017.06.013 | es_ES |
dc.identifier.issn | 0981-9428 (Print) | - |
dc.identifier.issn | 1873-2690 (Online) | - |
dc.identifier.uri | http://hdl.handle.net/10045/67477 | - |
dc.description.abstract | Transplastomic plants are a system of choice for the mass production of biopharmaceuticals due to the polyploidy of the plastid genome and the low risk of pollen-mediated outcrossing because of maternal inheritance. However, as field-grown plants, they can suffer contamination by agrochemicals and fertilizers, as well as fluctuations in yield due to climatic changes and infections. Tissue-type plasminogen activator (tPA), a protein used to treat heart attacks, converts plasminogen into plasmine, which digests fibrin and induces the dissolution of fibrin clots. Recently, we obtained transplastomic tobacco plants carrying the K2S gene encoding truncated human tPA (reteplase) with improved biological activity, and confirmed the presence of the target protein in the transgenic plant leaves. Considering the advantages of plant cell cultures for biopharmaceutical production, we established a cell line derived from the K2S tobacco plants. The active form of reteplase was quantified in cultures grown in light or darkness, with production 3-fold higher in light. | es_ES |
dc.description.sponsorship | This work has been supported by a grant from the Spanish Ministry of Science and Innovation (BIO2014-51861-R) and the Generalitat de Catalunya (2014SGR215). Diego Hidalgo is a predoctoral fellow of Mexican CONACyT. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | © 2017 Elsevier Masson SAS. | es_ES |
dc.subject | Tobacco cell cultures | es_ES |
dc.subject | Transplastomic plants | es_ES |
dc.subject | Human tissue plasminogen activator (tPA) | es_ES |
dc.subject | Reteplase | es_ES |
dc.subject.other | Bioquímica y Biología Molecular | es_ES |
dc.title | Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.peerreviewed | si | es_ES |
dc.identifier.doi | 10.1016/j.plaphy.2017.06.013 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.plaphy.2017.06.013 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2014-51861-R | - |
Aparece en las colecciones: | INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
---|---|---|---|---|
2017_Hidalgo_etal_PlantPhyBiochem_final.pdf | Versión final (acceso restringido) | 2,58 MB | Adobe PDF | Abrir Solicitar una copia |
2017_Hidalgo_etal_PlantPhyBiochem_accepted.pdf | Accepted Manuscript (acceso abierto) | 925,96 kB | Adobe PDF | Abrir Vista previa |
Todos los documentos en RUA están protegidos por derechos de autor. Algunos derechos reservados.