Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures

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Title: Biotechnological production of recombinant tissue plasminogen activator protein (reteplase) from transplastomic tobacco cell cultures
Authors: Hidalgo, Diego | Abdoli-Nasab, Maryam | Jalali-Javaran, Mokhtar | Bru-Martinez, Roque | Cusidó, Rosa M. | Corchete, Purificación | Palazon, Javier
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Tobacco cell cultures | Transplastomic plants | Human tissue plasminogen activator (tPA) | Reteplase
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: Sep-2017
Publisher: Elsevier
Citation: Plant Physiology and Biochemistry. 2017, 118: 130-137. doi:10.1016/j.plaphy.2017.06.013
Abstract: Transplastomic plants are a system of choice for the mass production of biopharmaceuticals due to the polyploidy of the plastid genome and the low risk of pollen-mediated outcrossing because of maternal inheritance. However, as field-grown plants, they can suffer contamination by agrochemicals and fertilizers, as well as fluctuations in yield due to climatic changes and infections. Tissue-type plasminogen activator (tPA), a protein used to treat heart attacks, converts plasminogen into plasmine, which digests fibrin and induces the dissolution of fibrin clots. Recently, we obtained transplastomic tobacco plants carrying the K2S gene encoding truncated human tPA (reteplase) with improved biological activity, and confirmed the presence of the target protein in the transgenic plant leaves. Considering the advantages of plant cell cultures for biopharmaceutical production, we established a cell line derived from the K2S tobacco plants. The active form of reteplase was quantified in cultures grown in light or darkness, with production 3-fold higher in light.
Sponsor: This work has been supported by a grant from the Spanish Ministry of Science and Innovation (BIO2014-51861-R) and the Generalitat de Catalunya (2014SGR215). Diego Hidalgo is a predoctoral fellow of Mexican CONACyT.
URI: http://hdl.handle.net/10045/67477
ISSN: 0981-9428 (Print) | 1873-2690 (Online)
DOI: 10.1016/j.plaphy.2017.06.013
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2017 Elsevier Masson SAS.
Peer Review: si
Publisher version: http://dx.doi.org/10.1016/j.plaphy.2017.06.013
Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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