Bioconversion of stilbenes in genetically engineered root and cell cultures of tobacco

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Títol: Bioconversion of stilbenes in genetically engineered root and cell cultures of tobacco
Autors: Hidalgo, Diego | Martínez Márquez, Ascensión | Moyano, Elisabeth | Bru-Martinez, Roque | Corchete, Purificación | Palazon, Javier
Grups d'investigació o GITE: Proteómica y Genómica Funcional de Plantas
Centre, Departament o Servei: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Paraules clau: Bioconversion | Stilbenes | Genetically engineered root | Cell cultures | Tobacco
Àrees de coneixement: Bioquímica y Biología Molecular
Data de publicació: 27-de març-2017
Editor: Springer Nature
Citació bibliogràfica: Scientific Reports. 2017, 7: 45331. doi:10.1038/srep45331
Resum: It is currently possible to transfer a biosynthetic pathway from a plant to another organism. This system has been exploited to transfer the metabolic richness of certain plant species to other plants or even to more simple metabolic organisms such as yeast or bacteria for the production of high added value plant compounds. Another application is to bioconvert substrates into scarcer or biologically more interesting compounds, such as piceatannol and pterostilbene. These two resveratrol-derived stilbenes, which have very promising pharmacological activities, are found in plants only in small amounts. By transferring the human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) gene to tobacco hairy roots and cell cultures, we developed a system able to bioconvert exogenous t-resveratrol into piceatannol in quantities near to mg L−1. Similarly, after heterologous expression of resveratrol O-methyltransferase from Vitis vinifera (VvROMT) in tobacco hairy roots, the exogenous t-resveratrol was bioconverted into pterostilbene. We also observed that both bioconversions can take place in tobacco wild type hairy roots (pRiA4, without any transgene), showing that unspecific tobacco P450 hydroxylases and methyltransferases can perform the bioconversion of t-resveratrol to give the target compounds, albeit at a lower rate than transgenic roots.
Patrocinadors: This work has been supported by a grant from the Spanish Ministry of Science and Innovation (BIO2014-51861-R), Generalitat de Catalunya (2014SGR215). Diego Hidalgo is a predoctoral fellow of Mexican CONACyT.
URI: http://hdl.handle.net/10045/65039
ISSN: 2045-2322
DOI: 10.1038/srep45331
Idioma: eng
Tipus: info:eu-repo/semantics/article
Drets: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Revisió científica: si
Versió de l'editor: http://dx.doi.org/10.1038/srep45331
Apareix a la col·lecció: INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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