A Focused Multiple Reaction Monitoring (MRM) Quantitative Method for Bioactive Grapevine Stilbenes by Ultra-High-Performance Liquid Chromatography Coupled to Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ)

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Title: A Focused Multiple Reaction Monitoring (MRM) Quantitative Method for Bioactive Grapevine Stilbenes by Ultra-High-Performance Liquid Chromatography Coupled to Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ)
Authors: Hurtado-Gaitán, Elías | Sellés Marchart, Susana | Martínez Márquez, Ascensión | Samper-Herrero, Antonio | Bru-Martinez, Roque
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: UHPLC | MRM | Resveratrol | Piceid | Piceatannol | Epsilon-viniferin | Pterostilbene | Cell culture | Bioconversion | Wine
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 7-Mar-2017
Publisher: MDPI
Citation: Hurtado-Gaitán E, Sellés-Marchart S, Martínez-Márquez A, Samper-Herrero A, Bru-Martínez R. A Focused Multiple Reaction Monitoring (MRM) Quantitative Method for Bioactive Grapevine Stilbenes by Ultra-High-Performance Liquid Chromatography Coupled to Triple-Quadrupole Mass Spectrometry (UHPLC-QqQ). Molecules. 2017; 22(3):418. doi:10.3390/molecules22030418
Abstract: Grapevine stilbenes are a family of polyphenols which derive from trans-resveratrol having antifungal and antimicrobial properties, thus being considered as phytoalexins. In addition to their diverse bioactive properties in animal models, they highlight a strong potential in human health maintenance and promotion. Due to this relevance, highly-specific qualitative and quantitative methods of analysis are necessary to accurately analyze stilbenes in different matrices derived from grapevine. Here, we developed a rapid, sensitive, and specific analysis method using ultra-high-performance liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC-QqQ) in MRM mode to detect and quantify five grapevine stilbenes, trans-resveratrol, trans-piceid, trans-piceatannol, trans-pterostilbene, and trans-ε-viniferin, whose interest in relation to human health is continuously growing. The method was optimized to minimize in-source fragmentation of piceid and to avoid co-elution of cis-piceid and trans-resveratrol, as both are detected with resveratrol transitions. The applicability of the developed method of stilbene analysis was tested successfully in different complex matrices including cellular extracts of Vitis vinifera cell cultures, reaction media of biotransformation assays, and red wine.
Sponsor: A.M.-M. acknowledges a grant from Conselleria d’Educacio, Cultura I Sport de la Generalitat Valenciana (FPA/2013/A/074). This work has been supported by grants from University of Alicante (VIGROB-105), the Spanish Ministry of Economy and Competitiveness (BIO2014-51861-R), and European funds for Regional development (FEDER).
URI: http://hdl.handle.net/10045/64367
ISSN: 1420-3049
DOI: 10.3390/molecules22030418
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Peer Review: si
Publisher version: http://dx.doi.org/10.3390/molecules22030418
Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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