CAZyme content of Pochonia chlamydosporia reflects that chitin and chitosan modification are involved in nematode parasitism
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Title: | CAZyme content of Pochonia chlamydosporia reflects that chitin and chitosan modification are involved in nematode parasitism |
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Authors: | Aranda-Martínez, Almudena | Lenfant, Nicolas | Escudero Benito, Nuria | Zavala-González, Ernesto A. | Henrissat, Bernard | Lopez-Llorca, Luis Vicente |
Research Group/s: | Fitopatología |
Center, Department or Service: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef" |
Keywords: | CAZymes | Chitin deacetylases | Chitosanases | Nematode egg parasitism nematophagous fungi | Pochonia chlamydosporia |
Knowledge Area: | Botánica |
Issue Date: | Nov-2016 |
Publisher: | John Wiley & Sons |
Citation: | Environmental Microbiology. 2016, 18(11): 4200-4215. doi:10.1111/1462-2920.13544 |
Abstract: | Pochonia chlamydosporia is a soil fungus with a multitrophic lifestyle combining endophytic and saprophytic behaviors, in addition to a nematophagous activity directed against eggs of root-knot and other plant parasitic nematodes. The carbohydrate-active enzymes encoded by the genome of P. chlamydosporia suggest that the endophytic and saprophytic lifestyles make use of a plant cell wall polysaccharide degradation machinery that can target cellulose, xylan and, to a lesser extent, pectin. This enzymatic machinery is completed by a chitin breakdown system that involves not only chitinases, but also chitin deacetylases and a large number of chitosanases. P. chlamydosporia can degrade and grow on chitin and is particularly efficient on chitosan. The relevance of chitosan breakdown during nematode egg infection is supported by the immunolocalization of chitosan in Meloidogyne javanica eggs infected by P. chlamydosporia and by the fact that the fungus expresses chitosanase and chitin deacetylase genes during egg infection. This suggests that these enzymes are important for the nematophagous activity of the fungus and they are targets for improving the capabilities of P. chlamydosporia as a biocontrol agent in agriculture. |
Sponsor: | This research was funded by the Spanish Ministry of Economy and Competitiveness Grant AGL2015-66833-R, with a grant from the Generalitat Valenciana to A. Aranda-Martinez (ACIF/2013/120) as well as a sabbatical grant to L.V. Lopez-Llorca (PR2015-0008). |
URI: | http://hdl.handle.net/10045/60257 |
ISSN: | 1462-2912 (Print) | 1462-2920 (Online) |
DOI: | 10.1111/1462-2920.13544 |
Language: | eng |
Type: | info:eu-repo/semantics/article |
Rights: | © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd |
Peer Review: | si |
Publisher version: | http://dx.doi.org/10.1111/1462-2920.13544 |
Appears in Collections: | INV - Fitopatología - Artículos de Revistas |
Files in This Item:
File | Description | Size | Format | |
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2016_Aranda_etal_EnvMicrob_final.pdf | Versión final (acceso restringido) | 1,62 MB | Adobe PDF | Open Request a copy |
2016_Aranda_etal_EnvMicrob_accepted.pdf | Accepted Manuscript (acceso abierto) | 1,79 MB | Adobe PDF | Open Preview |
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