CAZyme content of Pochonia chlamydosporia reflects that chitin and chitosan modification are involved in nematode parasitism

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Title: CAZyme content of Pochonia chlamydosporia reflects that chitin and chitosan modification are involved in nematode parasitism
Authors: Aranda-Martínez, Almudena | Lenfant, Nicolas | Escudero Benito, Nuria | Zavala-González, Ernesto A. | Henrissat, Bernard | Lopez-Llorca, Luis Vicente
Research Group/s: Fitopatología
Center, Department or Service: Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada | Universidad de Alicante. Instituto Multidisciplinar para el Estudio del Medio "Ramón Margalef"
Keywords: CAZymes | Chitin deacetylases | Chitosanases | Nematode egg parasitism nematophagous fungi | Pochonia chlamydosporia
Knowledge Area: Botánica
Issue Date: Nov-2016
Publisher: John Wiley & Sons
Citation: Environmental Microbiology. 2016, 18(11): 4200-4215. doi:10.1111/1462-2920.13544
Abstract: Pochonia chlamydosporia is a soil fungus with a multitrophic lifestyle combining endophytic and saprophytic behaviors, in addition to a nematophagous activity directed against eggs of root-knot and other plant parasitic nematodes. The carbohydrate-active enzymes encoded by the genome of P. chlamydosporia suggest that the endophytic and saprophytic lifestyles make use of a plant cell wall polysaccharide degradation machinery that can target cellulose, xylan and, to a lesser extent, pectin. This enzymatic machinery is completed by a chitin breakdown system that involves not only chitinases, but also chitin deacetylases and a large number of chitosanases. P. chlamydosporia can degrade and grow on chitin and is particularly efficient on chitosan. The relevance of chitosan breakdown during nematode egg infection is supported by the immunolocalization of chitosan in Meloidogyne javanica eggs infected by P. chlamydosporia and by the fact that the fungus expresses chitosanase and chitin deacetylase genes during egg infection. This suggests that these enzymes are important for the nematophagous activity of the fungus and they are targets for improving the capabilities of P. chlamydosporia as a biocontrol agent in agriculture.
Sponsor: This research was funded by the Spanish Ministry of Economy and Competitiveness Grant AGL2015-66833-R, with a grant from the Generalitat Valenciana to A. Aranda-Martinez (ACIF/2013/120) as well as a sabbatical grant to L.V. Lopez-Llorca (PR2015-0008).
ISSN: 1462-2912 (Print) | 1462-2920 (Online)
DOI: 10.1111/1462-2920.13544
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd
Peer Review: si
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Appears in Collections:INV - Fitopatología - Artículos de Revistas

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