New insights into plant salt acclimation: the roles of vesicle trafficking and reactive oxygen species signalling in mitochondria and the endomembrane system

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Title: New insights into plant salt acclimation: the roles of vesicle trafficking and reactive oxygen species signalling in mitochondria and the endomembrane system
Authors: Garcia de la Garma, Jesus | Fernandez-Garcia, Nieves | Bardisi, Enas | Pallol, Beatriz | Asensio-Rubio, Jose Salvador | Bru-Martinez, Roque | Olmos, Enrique
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Endoplasmic reticulum | Mitochondria | Nicotiana tabacum | Proline | Reactive oxygen species (ROS) | Salt acclimation | Vesicle trafficking
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: Jan-2015
Publisher: Wiley
Citation: New Phytologist. 2015, 205: 216–239. doi:10.1111/nph.12997
Abstract: In this study, we investigated the cellular and molecular mechanisms that regulate salt acclimation. The main objective was to obtain new insights into the molecular mechanisms that control salt acclimation. Therefore, we carried out a multidisciplinary study using proteomic, transcriptomic, subcellular and physiological techniques. We obtained a Nicotiana tabacum BY-2 cell line acclimated to be grown at 258 mM NaCl as a model for this study. The proteomic and transcriptomic data indicate that the molecular response to stress (chaperones, defence proteins, etc.) is highly induced in these salt-acclimated cells. The subcellular results show that salt induces sodium compartmentalization in the cell vacuoles and seems to be mediated by vesicle trafficking in tobacco salt-acclimated cells. Our results demonstrate that abscisic acid (ABA) and proline metabolism are crucial in the cellular signalling of salt acclimation, probably regulating reactive oxygen species (ROS) production in the mitochondria. ROS may act as a retrograde signal, regulating the cell response. The network of endoplasmic reticulum and Golgi apparatus is highly altered in salt-acclimated cells. The molecular and subcellular analysis suggests that the unfolded protein response is induced in salt-acclimated cells. Finally, we propose that this mechanism may mediate cell death in salt-acclimated cells.
Sponsor: The authors wish to thank Stephen Hasler for English language editing of the manuscript. This work was supported by the project BFU-2009-06499/AGR, from the Spanish Ministry of Education and Science (MEC-CICYT), and the project 08680/PI/08, from the SENECA foundation (Region de Murcia).
ISSN: 0028-646X (Print) | 1469-8137 (Online)
DOI: 10.1111/nph.12997
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2014 The Authors; New Phytologist Trust
Peer Review: si
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Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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