Microbiota Characterization of Compost Using Omics Approaches Opens New Perspectives for Phytophthora Root Rot Control

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Title: Microbiota Characterization of Compost Using Omics Approaches Opens New Perspectives for Phytophthora Root Rot Control
Authors: Blaya, Josefa | Marhuenda Egea, Frutos Carlos | Pascual, José Antonio | Ros, Margarita
Research Group/s: Grupo de Fotoquímica y Electroquímica de Semiconductores (GFES)
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Phytophthora root rot | Microbiota | Compost | Metagenomics | Metametabolomics
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 4-Aug-2016
Publisher: Public Library of Science (PLoS)
Citation: Blaya J, Marhuenda FC, Pascual JA, Ros M (2016) Microbiota Characterization of Compost Using Omics Approaches Opens New Perspectives for Phytophthora Root Rot Control. PLoS ONE 11(8): e0158048. doi:10.1371/journal.pone.0158048
Abstract: Phytophthora root rot caused by Phytophthora nicotianae is an economically important disease in pepper crops. The use of suppressive composts is a low environmental impact method for its control. Although attempts have been made to reveal the relationship between microbiota and compost suppressiveness, little is known about the microorganisms associated with disease suppression. Here, an Ion Torrent platform was used to assess the microbial composition of composts made of different agro-industrial waste and with different levels of suppressiveness against P. nicotianae. Both bacterial and fungal populations responded differently depending on the chemical heterogeneity of materials used during the composting process. High proportions (67–75%) of vineyard pruning waste were used in the most suppressive composts, COM-A and COM-B. This material may have promoted the presence of higher relative abundance of Ascomycota as well as higher microbial activity, which have proved to be essential for controlling the disease. Although no unique fungi or bacteria have been detected in neither suppressive nor conducive composts, relatively high abundance of Fusarium and Zopfiella were found in compost COM-B and COM-A, respectively. To the best of our knowledge, this is the first work that studies compost metabolome. Surprisingly, composts and peat clustered together in principal component analysis of the metabolic data according to their levels of suppressiveness achieved. This study demonstrated the need for combining the information provided by different techniques, including metagenomics and metametabolomics, to better understand the ability of compost to control plant diseases.
Sponsor: This work was supported by LIFE project AGROWASTE (LIFE10/ENV/ES/469) http://ec.europa.eu/environment/life/.
URI: http://hdl.handle.net/10045/57477
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0158048
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2016 Blaya et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Peer Review: si
Publisher version: http://dx.doi.org/10.1371/journal.pone.0158048
Appears in Collections:INV - GFES - Artículos de Revistas

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