Assessment of Visual and Chromatic Functions in a Rodent Model of Retinal Degeneration

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Title: Assessment of Visual and Chromatic Functions in a Rodent Model of Retinal Degeneration
Authors: Segura, Francisco | Sánchez Cano, Ana | Jarabo, Sebastián | López de la Fuente, Carmen | Cuenca, Nicolás | Villegas Pérez, María Paz | Pinilla Lozano, Isabel
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: P23H | Retinitis pigmentosa | Optokinetic tracking | Electroretinogram
Knowledge Area: Biología Celular
Issue Date: Oct-2015
Publisher: Association for Research in Vision and Ophthalmology (ARVO)
Citation: Investigative Ophthalmology & Visual Science. 2015, 56(11): 6275-6283. doi:10.1167/iovs.15-17257
Abstract: Purpose: We evaluated the photoreceptor response of pigmented P23H and normal pigmented Long Evans (LE) rats over time using functional tests in variable lighting conditions. Methods: Pigmented P23H rats were studied by optomotor testing and electroretinogram (ERG) recordings at P30, P150, and P240. Pigmented LE rats were used as a normal wild-type control. Stimuli were modified with colored filters. Neutral density filters were used to reduce luminance. Results: Age-related decreases in visual acuity (VA) and contrast sensitivity (CS) were observed in P23H rats. Good correlations in measurements without filter and with green filter were observed between LE and P23H P30 rat values. Differences between groups were smaller with red and purple filters. A strong relationship with luminance was observed in LE rats (VA and CS) and with P23H P30 rats (CS). A decline in the ERG responses of P23H rats was consistent with the gradual loss of photoreceptors. Differences in a- and b-wave amplitudes with different colored filters were negligible with the exception of the red filter, which resulted in smaller responses. Conclusions: Visual function parameters decreased with age in pigmented P23H rats. Irrespective of luminance, color filter, and retinal degeneration, minimum thresholds of VA and CS were found. Smaller differences than expected were found using color filters. Responses to functional tests at long wavelengths were observed, where there is very low photoreceptor spectral sensitivity. The use of filters with functional testing could minimize light-induced retinal damage in rats.
Sponsor: Supported by the Spanish Ministry of Health ISCIII (PI13/01124, PS0901854), DGA Group B99, ISCIII RETICS RD12/0034/0010, and by a Zaragoza University Grant FPUZ-2011-BIO-02 (FS).
ISSN: 0146-0404 (Print) | 1552-5783 (Online)
DOI: 10.1167/iovs.15-17257
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2015 The Association for Research in Vision and Ophthalmology, Inc.
Peer Review: si
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Appears in Collections:INV - NEUROVIS - Artículos de Revistas

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