Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase

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Title: Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase
Authors: Santamaría-Gómez, Javier | Ochoa de Alda, Jesús A.G. | Olmedo-Verd, Elvira | Bru-Martinez, Roque | Luque Romero, Ignacio
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Aminoacyl-tRNA synthetases | Membrane-anchoring | Cyanobacteria | FoF1 ATP synthase | Thylakoids | CAAD
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 6-Jun-2016
Publisher: Frontiers Media
Citation: Santamaría-Gómez J, Ochoa de Alda JAG, Olmedo-Verd E, Bru-Martínez R and Luque I (2016) Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase. Front. Microbiol. 7:857. doi: 10.3389/fmicb.2016.00857
Abstract: tRNAs are charged with cognate amino acids by aminoacyl-tRNA synthetases (aaRSs) and subsequently delivered to the ribosome to be used as substrates for gene translation. Whether aminoacyl-tRNAs are channeled to the ribosome by transit within translational complexes that avoid their diffusion in the cytoplasm is a matter of intense investigation in organisms of the three domains of life. In the cyanobacterium Anabaena sp. PCC 7120, the valyl-tRNA synthetase (ValRS) is anchored to thylakoid membranes by means of the CAAD domain. We have investigated whether in this organism ValRS could act as a hub for the nucleation of a translational complex by attracting other aaRSs to the membranes. Out of the 20 aaRSs, only ValRS was found to localize in thylakoid membranes whereas the other enzymes occupied the soluble portion of the cytoplasm. To investigate the basis for this asymmetric distribution of aaRSs, a global search for proteins interacting with the 20 aaRSs was conducted. The interaction between ValRS and the FoF1 ATP synthase complex here reported is of utmost interest and suggests a functional link between elements of the gene translation and energy production machineries.
Sponsor: This work was supported by grants BFU2010-19544 and BFU2013-44686-P from Ministerio de Economía y Competitividad of Spain and FEDER.
URI: http://hdl.handle.net/10045/55589
ISSN: 1664-302X
DOI: 10.3389/fmicb.2016.00857
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2016 Santamaría-Gómez, Ochoa de Alda, Olmedo-Verd, Bru-Martínez and Luque. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Peer Review: si
Publisher version: http://dx.doi.org/10.3389/fmicb.2016.00857
Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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