Denitrification Enzymes-Based Biosensors: the Case of Haloarchaeal Enzymes

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Title: Denitrification Enzymes-Based Biosensors: the Case of Haloarchaeal Enzymes
Authors: Torregrosa-Crespo, Javier | Pire, Carmen | Martínez-Espinosa, Rosa María
Research Group/s: Biotecnología de Extremófilos (BIOTECEXTREM)
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Biosensors | Enzymes | Denitrification | Respiratory nitrate reductase | Respiratory nitrite reductase
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: Apr-2016
Publisher: IJABPT
Citation: International Journal of Applied Biology and Pharmaceutical Technology. 2016, 7(2): 285-289
Abstract: In the last two decades, the increase in the use of artificial fertilizers and the disposal of industrial wastes have been the main factors responsible for the progressive increase in nitrate and nitrite levels in groundwater and soil. A variety of analytical strategies have been developed for nitrate and nitrite detection but electrochemical biosensors, which are simple, cheap, easily miniaturized and suitability for real-time detection, are proved to be a powerful tool. Various types of biosensors based on the use of whole cells or on the immobilization of denitrification enzymes have been developed, but their use is limited in environmental analysis under extreme conditions such as brines, acidic or basic wastewaters, salted soils, etc. Extremophilic denitrifying microorganism are good candidates for the development of new nitrate and nitrite biosensors and, in particular, haloarchaeal based biosensors would have advantages over bacterial based biosensors since the microorganisms and the purified denitrifying enzymes tolerate a wide range of temperature and salinity. This work summarizes new highlights on the potential uses of denitrifying haloarchaeal enzymes to make enzyme-based biosensors.
Sponsor: This work was funded by research grant from the MINECO Spain (CTM2013-43147-R).
URI: http://hdl.handle.net/10045/54627
ISSN: 0976-4550
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2016 Rosa María Martínez-Espinosa. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Peer Review: si
Publisher version: http://www.ijabpt.com/Details.aspx?fid=1314
Appears in Collections:INV - BIOTECEXTREM - Artículos de Revistas
INV - AppBiochem - Artículos de Revistas

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