Effect of elite physical exercise by triathletes on seven catabolites of DNA oxidation
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Título: | Effect of elite physical exercise by triathletes on seven catabolites of DNA oxidation |
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Autor/es: | Villaño, Débora | Vilaplana, Cristina | Medina, Sonia | Cejuela, Roberto | Martínez Sanz, José Miguel | Gil, Pilar | Genieser, Hans-Gottfried | Ferreres, Federico | Gil-Izquierdo, Ángel |
Grupo/s de investigación o GITE: | Grupo de Investigación en Ciencias de la Actividad Física y el Deporte (GICAFD) | Grupo de Investigación en Alimentación y Nutrición (ALINUT) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Didáctica General y Didácticas Específicas | Universidad de Alicante. Departamento de Enfermería |
Palabras clave: | Oxidative stress | DNA oxidation biomarkers | Sport | 8-hydroxy-deoxyguanosine | 8-hydroxyguanine |
Área/s de conocimiento: | Educación Física y Deportiva | Enfermería |
Fecha de publicación: | 8-abr-2015 |
Editor: | Taylor & Francis |
Cita bibliográfica: | Free Radical Research. 2015, 49(8): 973-983. doi:10.3109/10715762.2015.1025388 |
Resumen: | The oxidized nucleoside 8-hydroxy-2’-deoxyguanosine has been widely studied as a marker of DNA oxidation; however, data on the occurrence of other metabolites in plasma that are related to DNA damage are scarce. We have applied an improved, sensitive, robust, and reliable method, involving solid phase extraction and ultrahigh-performance liquid chromatography (UHPLC)–tandem mass spectrometry (MS/MS), to the precise quantitation of seven metabolites in the plasma of 15 elite triathletes after a 2-week training program. All compounds were eluted in the first 1.6 min, with limits of detection and quantification ranging between 0.001 and 0.3 ng.mL−1 and 0.009 and 0.6 ng.mL−1, respectively. Four compounds were detected in plasma: guanosine-3’-5’-cyclic monophosphate, 8-hydroxyguanine, 8-hydroxy-2’-deoxyguanosine, and 8-nitroguanosine. After two weeks of training, 8-hydroxyguanine exhibited the highest increase (from 0.031 ± 0.008 nM to 0.036 ± 0.012 nM) (p < 0.05), which could be related to the enhanced activity of DNA-repairing enzymes that excise this oxidized base. Increased levels of guanosine-3’-5’-cyclic monophosphate and 8-hydroxy-2’-deoxyguanosine were also observed. In contrast, levels of 8-nitroguanosine (p < 0.05) were significantly reduced, which might be a protective measure as this compound strongly stimulates the generation of superoxide radicals, and its excess is related to pathologies such as microbial (viral) infections and other inflammatory and degenerative disorders. The results obtained indicate an induced adaptive response to the increased oxidative stress related to elite training, and point to the benefits associated with regular exercise. |
Patrocinador/es: | The study received financial support from the national funding agencies through the projects AGL2011-23690 (CICYT), CSD007-0063 (CONSOLIDER-INGENIO 2010 “Fun-c-Food”), and CSIC 201170E041 (Spanish Ministry of Economy and Competitiveness). Sonia Medina is grateful to the CICYT for a research contract (AGL2011-23690). |
URI: | http://hdl.handle.net/10045/53377 |
ISSN: | 1071-5762 (Print) | 1029-2470 (Online) |
DOI: | 10.3109/10715762.2015.1025388 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2015 Informa UK, Ltd. |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.3109/10715762.2015.1025388 |
Aparece en las colecciones: | INV - GICAFD - Artículos de Revistas INV - ALINUT - Artículos de Revistas INV - SCAPE - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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2015_Villano_etal_FreeRadicalRes_final.pdf | Versión final (acceso restringido) | 345,84 kB | Adobe PDF | Abrir Solicitar una copia |
2015_Villano_etal_FreeRadicalRes_accepted.pdf | Accepted Manuscript (acceso abierto) | 701,64 kB | Adobe PDF | Abrir Vista previa |
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