Assessment oxidative stress biomarkers –neuroprostanes and dihomo-isoprostanes- in elite triathletes urine after two weeks of moderate altitude training

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Title: Assessment oxidative stress biomarkers –neuroprostanes and dihomo-isoprostanes- in elite triathletes urine after two weeks of moderate altitude training
Authors: García-Flores, Libia Alejandra | Medina, Sonia | Cejuela Anta, Roberto | Martínez Sanz, José Miguel | Oger, Camille | Galano, Jean-Marie | Durand, Thierry | Casas-Pina, Teresa | Martínez-Hernández, Pedro | Ferreres, Federico | Gil-Izquierdo, Ángel
Research Group/s: Grupo de Investigación en Ciencias de la Actividad Física y el Deporte (GICAFD) | Grupo de Investigación en Alimentación y Nutrición (ALINUT)
Center, Department or Service: Universidad de Alicante. Departamento de Didáctica General y Didácticas Específicas | Universidad de Alicante. Departamento de Enfermería
Keywords: Neuroprostanes | Dihomo-isoprostanes | Oxidative stress | Altitude | Physical exercise | UHPLC-QqQ-MS/MS
Knowledge Area: Educación Física y Deportiva | Enfermería
Issue Date: 2015
Publisher: Taylor & Francis
Citation: Free Radical Research. 2015. doi:10.3109/10715762.2015.1111514
Abstract: This randomized and controlled trial investigated whether the increase in elite training at different altitudes altered the oxidative stress biomarkers of the nervous system. This is the first study to investigate four F4-neuroprostanes and four F2-dihomo-isoprostanes quantified in 24-hour urine. The quantification was carried out by Ultra High Pressure Liquid Chromatography-triple Quadrupole-Tandem Mass Spectrometry (UHPLC-QqQ-MS/MS). Sixteen elite triathletes agreed to participate in the project. They were randomized in two groups, a group submitted to Altitude Training (n=8) and a group submitted to Sea Level Training (n=8), with a Control group of non-athletes (n=8). After experimental period, the Altitude Training group triathletes gave significant data: 17-epi-17-F2t-dihomo-IsoP (from 5.2 ± 1.4 µg/mL 24 h-1 to 6.6 ± 0.6 µg/mL 24 h-1), ent-7(RS)-7-F2t-dihomo-IsoP (from 6.6 ± 1.7 µg/mL 24 h-1 to 8.6 ± 0.9 µg /mL 24 h-1), and ent-7-epi-7-F2t-dihomo-IsoP (from 8.4 ± 2.2 µg/mL 24 h-1 to 11.3 ± 1.8 µg/mL 24 h-1) increased, while, of the neuronal degeneration-related compounds, only 10-epi-10-F4t-NeuroP (8.4 ± 1.7 µg/mL 24 h-1) and 10-F4t-NeuroP (5.2 ± 2.9 µg/mL 24 h-1) were detected in this group. For the control group and sea level training groups, no significant changes had occurred at the end of the 2-weeks experimental period. Therefore, and as the main conclusion, the training at moderate altitude increased the F4-NeuroPs- and F2-dihomo-isoPs-related oxidative damage of the central nervous system (CNS) compared to similar training at sea level.
Sponsor: This study was supported by the project AGL2011-23690 (CICYT) (Spanish Ministry of Economy and Competitiveness). LAGF was granted with a pre-doctoral FPI fellowship BES2012-060185 by the Spanish government. The authors are also grateful to the University of Alicante for its collaboration. Sonia Medina was appointed under a research contract from the project AGL2011-23690 (CICYT).
URI: http://hdl.handle.net/10045/53376
ISSN: 1071-5762 (Print) | 1029-2470 (Online)
DOI: 10.3109/10715762.2015.1111514
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2015 Taylor & Francis
Peer Review: si
Publisher version: http://dx.doi.org/10.3109/10715762.2015.1111514
Appears in Collections:INV - ALINUT - Artículos de Revistas
INV - GICAFD - Artículos de Revistas
INV - RIPEFAP - Artículos de Revistas

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