Transcriptional profiles of Haloferax mediterranei based on nitrogen availability

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Title: Transcriptional profiles of Haloferax mediterranei based on nitrogen availability
Authors: Esclapez Espliego, Julia María | Pire, Carmen | Camacho, Mónica | Bautista, Vanesa | Martínez-Espinosa, Rosa María | Zafrilla Requena, Basilio | Vegara Luque, Anna | Alcaraz, Luis A. | Bonete, María-José
Research Group/s: Biotecnología de Extremófilos (BIOTECEXTREM)
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Nitrogen assimilation | Archaea | DNA microarray | Transcriptomic analysis
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 10-Jan-2015
Publisher: Elsevier
Citation: Journal of Biotechnology. 2015, 193: 100-107. doi:10.1016/j.jbiotec.2014.11.018
Abstract: The haloarchaeon Haloferax mediterranei is able to grow in the presence of different inorganic and organic nitrogen sources by means of the assimilatory pathway under aerobic conditions. In order to identify genes of potential importance in nitrogen metabolism and its regulation in the halophilic microorganism, we have analysed its global gene expression in three culture media with different nitrogen sources: (a) cells were grown stationary and exponentially in ammonium, (b) cells were grown exponentially in nitrate, and (c) cells were shifted to nitrogen starvation conditions. The main differences in the transcriptional profiles have been identified between the cultures with ammonium as nitrogen source and the cultures with nitrate or nitrogen starvation, supporting previous results which indicate the absence of ammonium as the factor responsible for the expression of genes involved in nitrate assimilation pathway. The results have also permitted the identification of transcriptional regulators and changes in metabolic pathways related to the catabolism and anabolism of amino acids or nucleotides. The microarray data was validated by real-time quantitative PCR on 4 selected genes involved in nitrogen metabolism. This work represents the first transcriptional profiles study related to nitrogen assimilation metabolism in extreme halophilic microorganisms using microarray technology.
Sponsor: This work was supported by the Spanish Ministry of Science and Innovation (MICINN, Grant BIO2008-00082), the Spanish Ministry of Economy and Competitivity (MINECO, Grant BIO2013-42921-P) and by project AEST/2013/062 from the Generalitat Valenciana (Spain).
URI: http://hdl.handle.net/10045/52854
ISSN: 0168-1656 (Print) | 1873-4863 (Online)
DOI: 10.1016/j.jbiotec.2014.11.018
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2014 Elsevier B.V.
Peer Review: si
Publisher version: http://dx.doi.org/10.1016/j.jbiotec.2014.11.018
Appears in Collections:INV - BIOTECEXTREM - Artículos de Revistas
INV - AppBiochem - Artículos de Revistas

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