In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

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Título: In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant
Autor/es: Marco Medina, Ana | Casas, Jose L.
Grupo/s de investigación o GITE: Biodiversidad y Biotecnología aplicadas a la Biología de la Conservación
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Ciencias Ambientales y Recursos Naturales | Universidad de Alicante. Centro Iberoamericano de la Biodiversidad
Palabras clave: Micropropagation | Essential oil | In vitro culture | Thymus | Double-phase culture system
Área/s de conocimiento: Fisiología Vegetal
Fecha de publicación: ene-2015
Editor: Springer Science+Business Media Dordrecht
Cita bibliográfica: Plant Cell, Tissue and Organ Culture (PCTOC). 2015, 120(1): 99-108. doi:10.1007/s11240-014-0583-4
Resumen: Thymus moroderi Pau ex Martínez (Lamiaceae) is a species endemic to South-eastern Spain valuable for its use in traditional medicine and in the production of a liqueur called cantueso. We describe a rapid and reliable in vitro propagation protocol for this species, starting from wild adult vegetative explants. Sterilization and establishment of in vitro culture were shown to be extremely difficult because of the high presence of contamination and the inability of non-contaminated explants to restore growth. In just two cases we were able to obtain non-contaminated explants with ability to grow: combining a fungicide dip with the presence of kanamycin in culture medium, and when mother plants were maintained for a week in a growth chamber with occasional fungicide spray. Once established in vitro, explants showed rapid and vigorous growth with spontaneous rooting. The presence of cytokinin alone or in combination with auxin gave worse results than untreated controls, thus revealing Thymus moroderi as a cytokinin-sensitive species. Better multiplication rates were however obtained when double phase system was included in the protocol. Fully acclimatized micropropagated plants were phenotypically indistinguishable from their wild relatives. Essential oils of micropropagated and wild plants were analyzed revealing a slightly different overall terpene profile. Micropropagated plants were abundant in monoterpene and sesquiterpene hydrocarbons but less in oxygenated sesquiterpenes with respect to wild plants. Major compounds were in both cases 1,8-cineole and camphor, although at different relative abundances. Despite these dissimilarities micropropagated plants produced a clearly recognizable and characteristic thyme odor.
Patrocinador/es: A Marco-Medina was financially supported by a Predoctoral fellowship (FPU) from the Spanish Ministry of Science and Innovation.
URI: http://hdl.handle.net/10045/52817
ISSN: 0167-6857 (Print) | 1573-5044 (Online)
DOI: 10.1007/s11240-014-0583-4
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © Springer Science+Business Media Dordrecht 2014. The final publication is available at Springer via http://dx.doi.org/10.1007/s11240-014-0583-4
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1007/s11240-014-0583-4
Aparece en las colecciones:INV - BBaBC - Artículos de Revistas

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