Long time remodeling during retinal degeneration evaluated by optical coherence tomography, immunocytochemistry and fundus autofluorescence

Please use this identifier to cite or link to this item: http://hdl.handle.net/10045/52017
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Title: Long time remodeling during retinal degeneration evaluated by optical coherence tomography, immunocytochemistry and fundus autofluorescence
Authors: Pinilla Lozano, Isabel | Fernández Sánchez, Laura | Segura, Francisco | Sánchez Cano, Ana | Tamarit, José Manuel | Fuentes-Broto, Lorena | Eells, Janis T. | Lax, Pedro | Cuenca, Nicolás
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Retina | OCT | Immunocytochemistry | P23H | Retinitis pigmentosa | Remodeling
Knowledge Area: Biología Celular | Fisiología
Issue Date: 29-Oct-2015
Publisher: Elsevier
Citation: Experimental Eye Research. 2015. doi:10.1016/j.exer.2015.10.012
Abstract: Purpose: To characterize the relationship between fundus autofluorescence (FAF), Optical Coherence Tomography (OCT) and immunohistochemistry (IHC) over the course of chronic retinal degeneration in the P23H rat. Methods: Homozygous albino P23H rats, Sprague–Dawley (SD) rats as controls and pigmented Long Evans (LE) rats were used. A Spectralis HRA OCT system was used for scanning laser ophthalmoscopy (SLO) imaging OCT and angiography. To determine FAF, fluorescence was excited using diode laser at 488 nm. A fast retina map OCT was performed using the optic nerve as a landmark. IHC was performed to correlate with the findings of OCT and FAF changes. Results: During the course of retinal degeneration, the FAF pattern evolved from some spotting at 2 months old to a mosaic of hyperfluorescent dots in rats 6 months and older. Retinal thicknesses progressively diminished over the course of the disease. At later stages of degeneration, OCT documented changes in the retinal layers, however, IHC better identified the cell loss and remodeling changes. Angiography revealed attenuation of the retinal vascular plexus with time. Conclusion: We provide for the first time a detailed long-term analysis of the course of retinal degeneration in P23H rats using a combination of SLO and OCT imaging, angiography, FAF and IHC. Although, the application of noninvasive methods enables longitudinal studies and will decrease the number of animals needed for a study, IHC is still an essential tool to identify retinal changes at the cellular level.
Sponsor: Instituto de Salud Carlos III FIS PI13/01124, Spanish Ministry of Economy and Competitiveness-FEDER BFU2012-36845, RETICS RD12/0034/0010, FUNDALUCE, ONCE. DGA group B99, FS was supported by a Zaragoza University grant FPUZ-2011-BIO-02.
URI: http://hdl.handle.net/10045/52017
ISSN: 0014-4835 (Print) | 1096-0007 (Online)
DOI: 10.1016/j.exer.2015.10.012
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2015 Elsevier Ltd.
Peer Review: si
Publisher version: http://dx.doi.org/10.1016/j.exer.2015.10.012
Appears in Collections:INV - NEUROVIS - Artículos de Revistas

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