Organic distributed feedback laser for label-free biosensing of ErbB2 protein biomarker

Please use this identifier to cite or link to this item: http://hdl.handle.net/10045/50561
Información del item - Informació de l'item - Item information
Title: Organic distributed feedback laser for label-free biosensing of ErbB2 protein biomarker
Authors: Retolaza, Aritz | Martinez-Perdiguero, Josu | Merino, Santos | Morales-Vidal, Marta | Boj Giménez, Pedro | Quintana Arévalo, José Antonio | Villalvilla Soria, José Moisés | Díaz-García, María A.
Research Group/s: Física de la Materia Condensada
Center, Department or Service: Universidad de Alicante. Departamento de Óptica, Farmacología y Anatomía | Universidad de Alicante. Departamento de Física Aplicada | Universidad de Alicante. Instituto Universitario de Materiales
Keywords: Label-free biosensors | Organic lasers | Distributed feedback | Perylenediimides | ErbB2 biomarker
Knowledge Area: Óptica | Física Aplicada | Física de la Materia Condensada
Issue Date: Feb-2016
Publisher: Elsevier
Citation: Sensors and Actuators B: Chemical. 2016, 223: 261-265. doi:10.1016/j.snb.2015.09.093
Abstract: The human epidermal growth factor receptor 2 (ErbB2) protein plays an important role in human malignancies. Its overexpression has been recognized as a feature of a malignant cancerous phenotype in breast cancer cell lines, and has become one of the most widely investigated clinical indicators of breast, ovarian, gastrointestinal and lung cancers. In this work a vertically emitting organic distributed feedback (DFB) laser has been used to detect the ErbB2 protein. This DFB laser consists of a polystyrene (PS) film containing a perylenediimide laser dye, deposited over a second-order one dimensional grating fabricated on fused silica by thermal-nanoimprint lithography and subsequent reactive ion etching processes. Specificity of the system to ErbB2 protein biomarker, achieved by functionalizing the PS with anti-ErbB2 monoclonal antibodies, is demonstrated. A concentration limit of detection for ErbB2 protein of 14 ng/mL has been obtained, and no cross-reactivity has been observed with bovine serum albumin (BSA) and tumor necrosis factor alpha (TNFα) proteins. These findings open the possibility of using this type of biosensors in clinical applications.
Sponsor: This work was supported by the Spanish Government (MINECO) and the European Community (FEDER) through grant no. MAT-2011–28167-C02. This work was partially funded by the Basque Government within the framework of the Etortek Program (Grant No. IE13-360). M. Morales-Vidal has been partly supported by a MINECO FPI fellowship (no. BES-2009-020747).
URI: http://hdl.handle.net/10045/50561
ISSN: 0925-4005 (Print) | 1873-3077 (Online)
DOI: 10.1016/j.snb.2015.09.093
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2015 Elsevier B.V.
Peer Review: si
Publisher version: http://dx.doi.org/10.1016/j.snb.2015.09.093
Appears in Collections:INV - Física de la Materia Condensada - Artículos de Revistas
INV - GHPO - Artículos de Revistas

Files in This Item:
Files in This Item:
File Description SizeFormat 
Thumbnail2016_Retolaza_etal_SensorsB_final.pdfVersión final (acceso restringido)637,21 kBAdobe PDFOpen    Request a copy
Thumbnail2016_Retolaza_etal_SensorsB_accepted.pdfAccepted Manuscript (acceso abierto)161,32 kBAdobe PDFOpen Preview


Items in RUA are protected by copyright, with all rights reserved, unless otherwise indicated.