The role of proteomics in progressing insights into plant secondary metabolism

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Title: The role of proteomics in progressing insights into plant secondary metabolism
Authors: Martínez Esteso, María José | Martínez Márquez, Ascensión | Sellés Marchart, Susana | Morante Carriel, Jaime | Bru-Martinez, Roque
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Secondary metabolism | Polyphenols | Alkaloids | Terpenoids | Cell cultures | Shotgun proteomics | DIGE | MRM
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 7-Jul-2015
Publisher: Frontiers Media
Citation: Martínez-Esteso MJ, Martínez-Márquez A, Sellés-Marchart S, Morante-Carriel JA and Bru-Martínez R (2015) The role of in proteomics progressing insights into plant secondary metabolism. Front. Plant Sci. 6:504 doi:10.3389/fpls.2015.00504
Abstract: The development of omics has enabled the genome-wide exploration of all kinds of biological processes at the molecular level. Almost every field of plant biology has been analyzed at the genomic, transcriptomic and proteomic level. Here we focus on the particular contribution that proteomic technologies have made in progressing knowledge and characterising plant secondary metabolism (SM) pathways since early expectations were created 15 years ago. We analyzed how three major issues in the proteomic analysis of plant SM have been implemented in various research studies. These issues are: (i) the selection of a suitable plant material rich in secondary metabolites of interest, such as specialized tissues and organs, and in vitro cell cultures; (ii) the proteomic strategy to access target proteins, either a comprehensive or a differential analysis; (iii) the proteomic approach, represented by the hypothesis-free discovery proteomics and the hypothesis-driven targeted proteomics. We also examine to what extent the most-advanced technologies have been incorporated into proteomic research in plant SM and highlight some cutting edge techniques that would strongly benefit the progress made in this field.
Sponsor: This work has been supported by grants from the Spanish Ministry of Science and Innovation (BIO2011-29856-C02-02; BIO2014-51861-R), European Funds for Regional Development (FEDER) and Conselleria d’Educacio, Cultura i Sport de la Generalitat Valenciana (FPA/2013/A/074). JM-C acknowledges a postdoctoral and research grants from SENESCYT GOVERNMENT OF ECUADOR (006-IECE-SMG5-GPLR-2012 and Programa1_Senescyt_2014) and a grant from UTEQ (UTEQ-Ambiental-9-FCAmb-IFOR-2014-FOCICYT002).
URI: http://hdl.handle.net/10045/48211
ISSN: 1664-462X
DOI: 10.3389/fpls.2015.00504
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2015 Martínez-Esteso, Martínez-Márquez, Sellés-Marchart, Morante-Carriel and Bru-Martínez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Peer Review: si
Publisher version: http://dx.doi.org/10.3389/fpls.2015.00504
Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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