Dissecting the Transcriptional Response to Elicitors in Vitis vinifera Cells

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Title: Dissecting the Transcriptional Response to Elicitors in Vitis vinifera Cells
Authors: Almagro, Lorena | Carbonell-Bejerano, Pablo | Belchí-Navarro, Sarai | Bru-Martinez, Roque | Martínez-Zapater, José M. | Lijavetzky, Diego | Pedreño, María A.
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Vitis vinifera cells | Transcriptional response | Elicitors
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 14-Oct-2014
Publisher: Public Library of Science (PLoS)
Citation: Almagro L, Carbonell-Bejerano P, Belchí-Navarro S, Bru R, Martínez-Zapater JM, Lijavetzky D, et al. (2014) Dissecting the Transcriptional Response to Elicitors in Vitis vinifera Cells. PLoS ONE 9(10): e109777. doi:10.1371/journal.pone.0109777
Abstract: The high effectiveness of cyclic oligosaccharides like cyclodextrins in the production of trans-resveratrol in Vitis vinifera cell cultures is enhanced in the presence of methyl jasmonate. In order to dissect the basis of the interactions among the elicitation responses triggered by these two compounds, a transcriptional analysis of grapevine cell cultures treated with cyclodextrins and methyl jasmonate separately or in combination was carried out. The results showed that the activation of genes encoding enzymes from phenylpropanoid and stilbene biosynthesis induced by cyclodextrins alone was partially enhanced in the presence of methyl jasmonate, which correlated with their effects on trans-resveratrol production. In addition, protein translation and cell cycle regulation were more highly repressed in cells treated with cyclodextrins than in those treated with methyl jasmonate, and this response was enhanced in the combined treatment. Ethylene signalling was activated by all treatments, while jasmonate signalling and salicylic acid conjugation were activated only in the presence of methyl jasmonate and cyclodextrins, respectively. Moreover, the combined treatment resulted in a crosstalk between the signalling cascades activated by cyclodextrins and methyl jasmonate, which, in turn, provoked the activation of additional regulatory pathways involving the up-regulation of MYB15, NAC and WRKY transcription factors, protein kinases and calcium signal transducers. All these results suggest that both elicitors cause an activation of the secondary metabolism in detriment of basic cell processes like the primary metabolism or cell division. Crosstalk between cyclodextrins and methyl jasmonate-induced signalling provokes an intensification of these responses resulting in a greater trans-resveratrol production.
Sponsor: This work was supported by the Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia en el marco de II PCTRM 2007-10 (08799/PI/08) and Ministerio de Economía y Competitividad-Fondo Europeo de Desarrollo Regional (BIO2008-03892 and BIO2011-29856-C02-02); Agencia Nacional de Promoción Científica y Tecnológica-MINCYT-Argentina (PAE-PICT 2360).
URI: http://hdl.handle.net/10045/47709
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0109777
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2014 Almagro et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Peer Review: si
Publisher version: http://dx.doi.org/10.1371/journal.pone.0109777
Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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