Determination of fat-soluble vitamins in vegetable oils through microwave-assisted high-performance liquid chromatography

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Title: Determination of fat-soluble vitamins in vegetable oils through microwave-assisted high-performance liquid chromatography
Authors: Carballo Marrero, Silvia | Prats Moya, Soledad | Maestre, Salvador E. | Todolí Torró, José Luis
Research Group/s: Análisis de Polímeros y Nanomateriales | Análisis de Alimentos
Center, Department or Service: Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología
Keywords: Microwaves | Phylloquinone | Tocopherols | Vegetable oils
Knowledge Area: Nutrición y Bromatología | Química Analítica
Issue Date: Apr-2015
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
Citation: Journal of Separation Science. 2015, 38(7): 1073-1081. doi:10.1002/jssc.201401262
Abstract: In this manuscript, a study of the effect of microwave radiation on the high-performance liquid chromatography separation of tocopherols and vitamin K1 was conducted. The novelty of the application was the use of a relatively low polarity mobile phase in which the dielectric heating effect was minimized to evaluate the nonthermal effect of the microwave radiation over the separation process. Results obtained show that microwave-assisted high-performance liquid chromatography had a shorter analysis time from 31.5 to 13.3 min when the lowest microwave power was used. Moreover, narrower peaks were obtained; hence the separation was more efficient maintaining or even increasing the resolution between the peaks. This result confirms that the increase in mobile phase temperature is not the only variable for improving the separation process but also other nonthermal processes must intervene. Fluorescence detection demonstrated better signal-to-noise compared to photodiode arrayed detection mainly due to the independent effect of microwave pulses on the baseline noise, but photodiode array detection was finally chosen as it allowed a simultaneous detection of nonfluorescent compounds. Finally, a determination of the content of the vitamin E homologs was carried out in different vegetable oils. Results were coherent with those found in the literature.
ISSN: 1615-9306 (Print) | 1615-9314 (Online)
DOI: 10.1002/jssc.201401262
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Peer Review: si
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Appears in Collections:INV - NANOBIOPOL - Artículos de Revistas
INV - AAQCN - Artículos de Revistas

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