Haloferax mediterranei GlnK proteins are post-translationally modified by uridylylation
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Título: | Haloferax mediterranei GlnK proteins are post-translationally modified by uridylylation |
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Autor/es: | Pedro Roig, Laia | Camacho, Mónica | Bonete, María-José |
Grupo/s de investigación o GITE: | Biotecnología de Extremófilos (BIOTECEXTREM) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Palabras clave: | Archaea | GlnK | Haloferax | Microbiology | Post-translational modification | Uridylylation |
Área/s de conocimiento: | Bioquímica y Biología Molecular |
Fecha de publicación: | abr-2013 |
Editor: | Wiley-VCH Verlag GmbH & Co. KGaA |
Cita bibliográfica: | Proteomics. 2013, 13(8): 1371-1374. doi:10.1002/pmic.201200465 |
Resumen: | In this work we report for the first time a post-translational modification of PII homologues from the Archaea Domain. Haloferax mediterranei is the first haloarchaea whose PII proteins have been studied, it possesses two of them (GlnK1 and GlnK2), both encoded adjacent to a gene for the ammonia transporter Amt. An approach based on 2DE, anti-GlnK immunoblot and peptide mass fingerprint (MALDI-TOF-MS) of the reactive spots showed that GlnK proteins in H. mediterranei are post-translationally uridylylated. A third spot with lower pI suggests the existence of a non-descript post-translational modification in this protein family. |
Patrocinador/es: | This work was supported by project BIO2008-00082 from the Spanish Ministry of Science and Innovation (MICINN), which includes funding from the European Union (“FEDER”). L.P.R. is supported by a fellowship (AP2007-02932) from the Ministry of Education. |
URI: | http://hdl.handle.net/10045/44400 |
ISSN: | 1615-9853 (Print) | 1615-9861 (Online) |
DOI: | 10.1002/pmic.201200465 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1002/pmic.201200465 |
Aparece en las colecciones: | INV - BIOTECEXTREM - Artículos de Revistas |
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2013_Pedro-Roig_etal_Proteomics_final.pdf | Versión final (acceso restringido) | 517,67 kB | Adobe PDF | Abrir Solicitar una copia |
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