Induction of trans-resveratrol and extracellular pathogenesis-related proteins in elicited suspension cultured cells of Vitis vinifera cv Monastrell
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Título: | Induction of trans-resveratrol and extracellular pathogenesis-related proteins in elicited suspension cultured cells of Vitis vinifera cv Monastrell |
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Autor/es: | Belchí-Navarro, Sarai | Almagro, Lorena | Sabater Jara, Ana Belén | Fernández Pérez, Francisco | Bru-Martinez, Roque | Pedreño, María A. |
Grupo/s de investigación o GITE: | Proteómica y Genómica Funcional de Plantas |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Palabras clave: | Cyclodextrins | Pathogenesis-related proteins | Signal molecules | Trans-resveratrol | Vitis vinifera |
Área/s de conocimiento: | Bioquímica y Biología Molecular |
Fecha de publicación: | 15-feb-2013 |
Editor: | Elsevier |
Cita bibliográfica: | Journal of Plant Physiology. 2013, 170(3): 258-264. doi:10.1016/j.jplph.2012.10.003 |
Resumen: | Suspension-cultured cells of Vitis vinifera cv Monastrell were used to investigate the effects of methyljasmonate, ethylene and salicylic acid separately or in combination with cyclodextrins on both trans-resveratrol production and the induction of defense responses. The results showed that the addition of methyljasmonate or ethylene to suspension-cultured cells jointly treated with cyclodextrins and salicylic acid provoked a decrease of trans-resveratrol levels suggesting that salicylic acid has a negative and antagonistic effect with methyljasmonate or ethylene on trans-resveratrol production. Likewise, the exogenous application of these compounds induced the accumulation of pathogenesis-related proteins. Analysis of the extracellular proteome showed the presence of amino acid sequences homologous to an specific β-1,3-glucanase, class III peroxidases and a β-1,4-mannanase, which suggests that these signal molecules could play a role in mediating defense-related gene product expression in V. vinifera cv Monastrell. Apart from these inducible proteins, other proteins were found in both the control and elicited cell cultures of V. vinifera. These included class IV chitinase, polygalacturonase inhibitor protein and reticuline oxidase-like protein, suggesting that their expression is constitutive being involved in the modification of the cell wall architecture during cell culture growth and in the prevention of pathogen attack. |
Patrocinador/es: | L. Almagro, AB, Sabater-Jara and F. Fernández-Pérez held a grant from the Ministerio de Ciencia e Innovación. This study was supported by the Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia en el marco de II PCTRM 2007-10 (08799/PI/08) and by MICINN-FEDER (BIO2008-2941 and BIO2011-29856-C02-02). |
URI: | http://hdl.handle.net/10045/40208 |
ISSN: | 0176-1617 (Print) | 1618-1328 (Online) |
DOI: | 10.1016/j.jplph.2012.10.003 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1016/j.jplph.2012.10.003 |
Aparece en las colecciones: | INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas |
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2013_Belchi_etal_JPP_final.pdf | Versión final (acceso restringido) | 375,7 kB | Adobe PDF | Abrir Solicitar una copia |
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