Induction of trans-resveratrol and extracellular pathogenesis-related proteins in elicited suspension cultured cells of Vitis vinifera cv Monastrell

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Title: Induction of trans-resveratrol and extracellular pathogenesis-related proteins in elicited suspension cultured cells of Vitis vinifera cv Monastrell
Authors: Belchí-Navarro, Sarai | Almagro, Lorena | Sabater Jara, Ana Belén | Fernández Pérez, Francisco | Bru-Martinez, Roque | Pedreño, María A.
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Cyclodextrins | Pathogenesis-related proteins | Signal molecules | Trans-resveratrol | Vitis vinifera
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 15-Feb-2013
Publisher: Elsevier
Citation: Journal of Plant Physiology. 2013, 170(3): 258-264. doi:10.1016/j.jplph.2012.10.003
Abstract: Suspension-cultured cells of Vitis vinifera cv Monastrell were used to investigate the effects of methyljasmonate, ethylene and salicylic acid separately or in combination with cyclodextrins on both trans-resveratrol production and the induction of defense responses. The results showed that the addition of methyljasmonate or ethylene to suspension-cultured cells jointly treated with cyclodextrins and salicylic acid provoked a decrease of trans-resveratrol levels suggesting that salicylic acid has a negative and antagonistic effect with methyljasmonate or ethylene on trans-resveratrol production. Likewise, the exogenous application of these compounds induced the accumulation of pathogenesis-related proteins. Analysis of the extracellular proteome showed the presence of amino acid sequences homologous to an specific β-1,3-glucanase, class III peroxidases and a β-1,4-mannanase, which suggests that these signal molecules could play a role in mediating defense-related gene product expression in V. vinifera cv Monastrell. Apart from these inducible proteins, other proteins were found in both the control and elicited cell cultures of V. vinifera. These included class IV chitinase, polygalacturonase inhibitor protein and reticuline oxidase-like protein, suggesting that their expression is constitutive being involved in the modification of the cell wall architecture during cell culture growth and in the prevention of pathogen attack.
Sponsor: L. Almagro, AB, Sabater-Jara and F. Fernández-Pérez held a grant from the Ministerio de Ciencia e Innovación. This study was supported by the Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia en el marco de II PCTRM 2007-10 (08799/PI/08) and by MICINN-FEDER (BIO2008-2941 and BIO2011-29856-C02-02).
ISSN: 0176-1617 (Print) | 1618-1328 (Online)
DOI: 10.1016/j.jplph.2012.10.003
Language: eng
Type: info:eu-repo/semantics/article
Peer Review: si
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Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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