Early signaling events in grapevine cells elicited with cyclodextrins and methyl jasmonate

Please use this identifier to cite or link to this item: http://hdl.handle.net/10045/40207
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Title: Early signaling events in grapevine cells elicited with cyclodextrins and methyl jasmonate
Authors: Belchí-Navarro, Sarai | Almagro, Lorena | Sabater Jara, Ana Belén | Fernández Pérez, Francisco | Bru-Martinez, Roque | Pedreño, María A.
Research Group/s: Proteómica y Genómica Funcional de Plantas
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Cyclodextrins | Elicitation | Hydrogen peroxide | Methyl jasmonate | Nitric oxide | Trans-resveratrol | Vitis vinifera cell cultures
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: Jan-2013
Publisher: Elsevier
Citation: Plant Physiology and Biochemistry. 2013, 62: 107-110. doi:10.1016/j.plaphy.2012.11.001
Abstract: The use of cyclic oligosaccharides like cyclodextrins (CDs), alone or combined with methyl jasmonate (MJ), as elicitors has proved very effective in stimulating the production of trans-resveratrol (trans-R) in Vitis vinifera suspension-cultured cells (SCC). Since elicitors can be used to increase trans-R production, understanding the molecular mechanisms involved would improve the management of grapevine cells as factories of this compound. The results obtained in this study provide evidence for a role of Ca2+ in mediating elicitor-induced trans-R production in grapevine SCC. The Ca2+ elevation was promoted by an uptake of Ca2+ from the extracellular medium, and by Ca2+ mobilization from intracellular organelles. Moreover, protein phosphorylation/dephosphorylation events seem to be involved in the signal transduction pathways triggered by CDs separately or in combination with MJ since trans-R production is dependent on both, the phosphorylation status of several proteins through mitogen-activated kinase pathway and the activity of tyrosine phosphatases. Our results also suggest that H2O2 and NO participated in the production of trans-R triggered by both elicitors in grapevine SCC. Finally, a fast alkalinization of the extracellular medium is induced in the presence of CDs and/or MJ.
Sponsor: L. Almagro, AB. Sabater-Jara and F. Fernández-Pérez have grants from the MICINN. This study was supported by the Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia en el marco de II PCTRM 2007e10 (08799/PI/08) and MICINN-FEDER (BIO2008-2941 and BIO2011-29856-C02-02).
URI: http://hdl.handle.net/10045/40207
ISSN: 0981-9428 (Print) | 1873-2690 (Online)
DOI: 10.1016/j.plaphy.2012.11.001
Language: eng
Type: info:eu-repo/semantics/article
Peer Review: si
Publisher version: http://dx.doi.org/10.1016/j.plaphy.2012.11.001
Appears in Collections:INV - Proteómica y Genómica Funcional de Plantas - Artículos de Revistas

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