Voltammetric behaviour of free DNA bases, methylcytosine and oligonucleotides at disposable screen printed graphite electrode platforms

Please use this identifier to cite or link to this item: http://hdl.handle.net/10045/38721
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Title: Voltammetric behaviour of free DNA bases, methylcytosine and oligonucleotides at disposable screen printed graphite electrode platforms
Authors: Brotons Cuevas, Ariadna | Alcaraz Mas, Luis Antonio | Metters, Jonathan P. | Banks, Craig E. | Iniesta, Jesus
Research Group/s: Electroquímica Aplicada y Electrocatálisis
Center, Department or Service: Universidad de Alicante. Departamento de Química Física | Universidad de Alicante. Instituto Universitario de Electroquímica
Keywords: Methylcytosine | Oligonucleotides | Free DNA bases | Screen printed graphite electrodes | Voltammetric behaviour
Knowledge Area: Química Física
Issue Date: 20-May-2013
Publisher: Royal Society of Chemistry
Citation: Analyst. 2013,138: 5239-5249. doi:10.1039/C3AN00682D
Abstract: Improvements in analytical methods for the determination and quantification of methylcytosine in DNA are vital since it has the potential to be used as a biomarker to detect different diseases in the first stage such as in the case of carcinomas and sterility. In this work we utilized screen printed graphite electrodes (SPGE) for studying the electrochemical response of all free DNA bases, methylcytosine and short oligonucleotides by cyclic voltammetry (CV) and square wave voltammetry (SWV). CV and SWV responses of free DNA bases and methylcytosine have been investigated by using SPGE platforms and the feasibility of detecting and quantifying cytosine and methylcytosine as free DNA moieties has been evaluated as a function of pH, concentration and the presence of the other free DNA bases in solution simultaneously. Repeatability of using SWV has been performed for the electrochemical behavior of both 250 μM cytosine and 250 μM methylcytosine in the presence of 25 μM guanine, with coefficient of variations of 6.9% and 2.6% respectively based upon peak current (N = 5). Six-mer oligonucleotides with a sequence 5′-XXXCGC-3′, where the XXX motif corresponds to TTT, TTA, TAA and AAA have been performed using SWV in 0.1 M acetate buffer pH 5.0 to explore how the DNA base position effects the electrooxidation of guanine and cytosine into the oligonucleotide. Furthermore SWV comparisons of the electrooxidation of the oligonucleotides 5′-CGCGCG-3′ and its methylated 5′-mCGmCGmCG-3′ have been performed with concentrations in acetate buffer solutions, and the interaction of both oligonucleotides with the graphitic surface of the SPGE has been demonstrated by fitting well-known adsorption models such as Freundlich and Langmuir kinetics according to the SWV current response of guanine, cytosine and methylcytosine into the oligonucleotide.
URI: http://hdl.handle.net/10045/38721
ISSN: 0003-2654 (Print) | 1364-5528 (Online)
DOI: 10.1039/C3AN00682D
Language: eng
Type: info:eu-repo/semantics/article
Rights: © Royal Society of Chemistry 2013
Peer Review: si
Publisher version: http://dx.doi.org/10.1039/C3AN00682D
Appears in Collections:INV - LEQA - Artículos de Revistas
INV - BIOTECEXTREM - Artículos de Revistas

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