Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli

Please use this identifier to cite or link to this item: http://hdl.handle.net/10045/33397
Información del item - Informació de l'item - Item information
Title: Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli
Authors: Almendros, Cristóbal | Guzmán, Noemí M. | Díez-Villaseñor, César | García-Martínez, Jesús | Mojica, Francisco J.M.
Research Group/s: Microbiología Molecular
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: CRISPR-Cas systems | Protospacer adjacent motif | Natural immunity | Escherichia coli
Knowledge Area: Microbiología
Issue Date: 26-Nov-2012
Publisher: Public Library of Science (PLoS)
Citation: ALMENDROS, Cristóbal, et al. “Target motifs affecting natural immunity by a constitutive CRISPR-Cas system in Escherichia coli”. PLoS ONE 7(11): e50797. doi:10.1371/journal.pone.0050797
Abstract: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated (cas) genes conform the CRISPR-Cas systems of various bacteria and archaea and produce degradation of invading nucleic acids containing sequences (protospacers) that are complementary to repeat intervening spacers. It has been demonstrated that the base sequence identity of a protospacer with the cognate spacer and the presence of a protospacer adjacent motif (PAM) influence CRISPR-mediated interference efficiency. By using an original transformation assay with plasmids targeted by a resident spacer here we show that natural CRISPR-mediated immunity against invading DNA occurs in wild type Escherichia coli. Unexpectedly, the strongest activity is observed with protospacer adjoining nucleotides (interference motifs) that differ from the PAM both in sequence and location. Hence, our results document for the first time native CRISPR activity in E. coli and demonstrate that positions next to the PAM in invading DNA influence their recognition and degradation by these prokaryotic immune systems.
Sponsor: This study was supported by the Spanish Ministerio de Ciencia e Innovación (BIO2011-24417).
URI: http://hdl.handle.net/10045/33397
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0050797
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2012 Almendros et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Peer Review: si
Publisher version: http://dx.doi.org/10.1371/journal.pone.0050797
Appears in Collections:INV - Microbiología Molecular - Artículos de Revistas

Files in This Item:
Files in This Item:
File Description SizeFormat 
Thumbnail2012_Almendros_etal_PLoS-ONE.pdf310,43 kBAdobe PDFOpen Preview


This item is licensed under a Creative Commons License Creative Commons