Rotenone induces degeneration of photoreceptors and impairs the dopaminergic system in the rat retina

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Title: Rotenone induces degeneration of photoreceptors and impairs the dopaminergic system in the rat retina
Authors: Esteve Rudd, Julián | Fernández Sánchez, Laura | Lax, Pedro | Juan Navarro, Emilio de | Martín-Nieto, José | Cuenca, Nicolás
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) | Genética Humana y de Mamíferos (GHM)
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Retina | Parkinson disease | Rotenone | Retinal degeneration | Pesticide | Mitochondria | Photoreceptor | Dopaminergic neuron
Knowledge Area: Biología Celular | Fisiología | Genética
Issue Date: 25-Jun-2011
Publisher: Elsevier
Citation: ESTEVE-RUDD, Julián, et al. “Rotenone induces degeneration of photoreceptors and impairs the dopaminergic system in the rat retina”. Neurobiology of Disease. Vol. 44, Issue 1 (Oct. 2011). ISSN 0969-9961, pp. 102-115
Abstract: Rotenone is a widely used pesticide and a potent inhibitor of mitochondrial complex I (NADH-quinone reductase) that elicits the degeneration of dopaminergic neurons and thereby the appearance of a parkinsonian syndrome. Here we have addressed the alterations induced by rotenone at the functional, morphological and molecular levels in the retina, including those involving both dopaminergic and non-dopaminergic retinal neurons. Rotenone-treated rats showed abnormalities in equilibrium, postural instability and involuntary movements. In their outer retina we observed a loss of photoreceptors, and a reduced synaptic connectivity between those remaining and their postsynaptic neurons. A dramatic loss of mitochondria was observed in the inner segments, as well as in the axon terminals of photoreceptors. In the inner retina we observed a decrease in the expression of dopaminergic cell molecular markers, including loss of tyrosine hydroxylase immunoreactivity, associated with a reduction of the dopaminergic plexus and cell bodies. An increase in immunoreactivity of AII amacrine cells for parvalbumin, a Ca2+-scavenging protein, was also detected. These abnormalities were accompanied by a decrease in the amplitude of scotopic and photopic a- and b-waves and an increase in the b-wave implicit time, as well as by a lower amplitude and greater latency in oscillatory potentials. These results indicate that rotenone induces loss of vision by promoting photoreceptor cell death and impairment of the dopaminergic retinal system.
Sponsor: This research was supported by grants from the Spanish Ministerio de Ciencia e Innovación (MICIN; BFU2009-07793/BFI), Instituto de Salud Carlos III, (RETICS RD07/0062/0012), Organización Nacional de Ciegos de España (ONCE), Fundación Lucha contra la Ceguera (FUNDALUCE) and Fundación Médica Mutua Madrileña.
URI: http://hdl.handle.net/10045/25069
ISSN: 0969-9961 (Print) | 1095-953X (Online)
DOI: 10.1016/j.nbd.2011.06.009
Language: eng
Type: info:eu-repo/semantics/article
Peer Review: si
Publisher version: http://dx.doi.org/10.1016/j.nbd.2011.06.009
Appears in Collections:INV - NEUROVIS - Artículos de Revistas
INV - GHM - Artículos de Revistas

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