Tauroursodeoxycholic acid prevents retinal degeneration in transgenic P23H rats

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Title: Tauroursodeoxycholic acid prevents retinal degeneration in transgenic P23H rats
Authors: Fernández Sánchez, Laura | Lax, Pedro | Pinilla Lozano, Isabel | Martín-Nieto, José | Cuenca, Nicolás
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS) | Genética Humana y de Mamíferos (GHM)
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Tauroursodeoxycholic acid | Retinal degeneration | Retinitis pigmentosa
Knowledge Area: Biología Celular | Fisiología | Genética
Issue Date: 1-Jul-2011
Publisher: Association for Research in Vision and Ophthalmology
Citation: FERNÁNDEZ-SÁNCHEZ, Laura, et al. "Tauroursodeoxycholic acid prevents retinal degeneration in transgenic P23H rats". Investigative Ophthalmology & Visual Science. Vol. 52, No. 8 (July 2011). ISSN 0146-0404, pp. 4998-5008
Abstract: Purpose. To evaluate the preventive effect of tauroursodeoxycholic acid (TUDCA) on photoreceptor degeneration, synaptic connectivity and functional activity of the retina in the transgenic P23H rat, an animal model of autosomal dominant retinitis pigmentosa (RP). Methods. P23H line-3 rats were injected with TUDCA once a week from postnatal day (P)21 to P120, in parallel with vehicle-administered controls. At P120, functional activity of the retina was evaluated by electroretinographic (ERG) recording. The effects of TUDCA on the number, morphology, integrity, and synaptic connectivity of retinal cells were characterized by immunofluorescence confocal microscopy. Results. The amplitude of ERG a- and b-waves was significantly higher in TUDCA-treated animals under both scotopic and photopic conditions than in control animals. In the central area of the retina, TUDCA-treated P23H rats showed threefold more photoreceptors than control animals. The number of TUNEL-positive cells was significantly smaller in TUDCA-treated rats, in which photoreceptor morphology was preserved. Presynaptic and postsynaptic elements, as well as the synaptic contacts between photoreceptors and bipolar or horizontal cells, were preserved in TUDCA-treated P23H rats. Furthermore, in TUDCA-treated rat retinas, the number of both rod bipolar and horizontal cell bodies, as well as the density of their synaptic terminals in the outer plexiform layer, was greater than in control rats. Conclusions. TUDCA treatment was capable of preserving cone and rod structure and function, together with their contacts with their postsynaptic neurons. The neuroprotective effects of TUDCA make this compound potentially useful for delaying retinal degeneration in RP.
Sponsor: Supported by grants from the Spanish Ministerio de Ciencia e Innovación (BFU2009-07793/BFI), Instituto de Salud Carlos III (RETICS RD07/0062/0012), Organización Nacional de Ciegos de España (ONCE), Fundación Lucha contra la Ceguera (FUNDALUCE), and Fundación de Investigación Médica Mutua Madrileña.
URI: http://hdl.handle.net/10045/25067
ISSN: 0146-0404 (Print) | 1552-5783 (Online)
DOI: 10.1167/iovs.11-7496
Language: eng
Type: info:eu-repo/semantics/article
Rights: Copyright © Association for Research in Vision and Ophthalmology
Peer Review: si
Publisher version: http://dx.doi.org/10.1167/iovs.11-7496
Appears in Collections:INV - NEUROVIS - Artículos de Revistas
INV - GHM - Artículos de Revistas

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