Functional and structural modifications during retinal degeneration in the rd10 mouse

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Title: Functional and structural modifications during retinal degeneration in the rd10 mouse
Authors: Barhoum, Rima | Martínez Navarrete, Gema Concepción | Corrochano Sánchez, Silvia | Germain Martínez, Francisco | Fernández Sánchez, Laura | Rosa Cano, Enrique J. de la | Villa Polo, Pedro de la | Cuenca, Nicolás
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Retina | Rod | Cone | Bipolar cells | Phosphodiesterase | Apoptosis
Knowledge Area: Oftalmología | Fisiología
Issue Date: 3-Jul-2008
Publisher: Elsevier
Citation: BARHOUM, Rima, et al. "Functional and structural modifications during retinal degeneration in the rd10 mouse". Neuroscience. Vol. 155, Issue 3 (26 Aug. 2008). ISSN 0306-4522, pp. 698-713
Abstract: Mouse models of retinal degeneration are useful tools to study therapeutic approaches for patients affected by hereditary retinal dystrophies. We have studied degener- ation in the rd10 mice both by immunocytochemistry and TUNEL-labeling of retinal cells, and through electrophysiological recordings. The cell degeneration in the retina of rd10 mice produced appreciable morphological changes in rod and cone cells by P20. Retinal cell death is clearly observed in the central retina and it peaked at P25 when there were 800 TUNEL-positive cells per mm2. In the central retina, only one row of photoreceptors remained in the outer nuclear layer by P40 and there was a remarkable deterioration of bipolar cell dendrites postsynaptic to photoreceptors. The axon terminals of bipolar cells also underwent atrophy and the inner retina was subject to further changes, including a reduction and disorganization of AII amacrine cell population. Glutamate sensitivity was tested in rod bipolar cells with the single cell patch-clamp technique in slice preparations, although at P60 no significant differences were observed with age-matched controls. Thus, we conclude that rod and cone degeneration in the rd10 mouse model is followed by deterioration of their postsynaptic cells and the cells in the inner retina. However, the functional preservation of receptors for photoreceptor transmission in bipolar cells may open new therapeutic possibilities.
Sponsor: This research was supported by grants from the Spanish Ministerio de Educación y Ciencia (SAF2004-05870 and SAF2007-66175 to E.J.d.l.R. and P.d.l.V., and BFI2003-01404 and BFU2006-00957 to N.C.), the Ministerio de Sanidad (RETICS RD07/0062 to P.d.l.V. and N.C.), the Comunidad de Madrid (08.5-0019.1/2001 to E.J.d.l.R. and 08.5– 0049/2003 to P.d.l.V.), Fundaluce to P.d.l.V., and the ONCE and Fundaluce to N.C. S.C. was supported by a postgraduate fellowship from the Ministerio de Educación y Ciencia.
URI: http://hdl.handle.net/10045/16723
ISSN: 0306-4522 (Print) | 1873-7544 (Online)
DOI: 10.1016/j.neuroscience.2008.06.042
Language: eng
Type: info:eu-repo/semantics/article
Peer Review: si
Publisher version: http://dx.doi.org/10.1016/j.neuroscience.2008.06.042
Appears in Collections:INV - NEUROVIS - Artículos de Revistas

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