Retinal degeneration in two lines of transgenic S334ter rats

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Title: Retinal degeneration in two lines of transgenic S334ter rats
Authors: Martínez Navarrete, Gema Concepción | Seiler, M.J. | Aramant, R.B. | Fernández-Sánchez, Laura | Pinilla Lozano, Isabel | Cuenca, Nicolás
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Retinal degeneration | Rodent | Remodeling | Apoptosis | Protein kinase C | Calbindin | Recoverin | Parvalbumin | Transducin
Knowledge Area: Oftalmología | Fisiología
Issue Date: 11-Dec-2010
Publisher: Elsevier
Citation: MARTÍNEZ NAVARRETE, Gema C., et al. "Retinal degeneration in two lines of transgenic S334ter rats". Experimental Eye Research. Vol. 92, No. 3 (March 2011). ISSN 0014-4835, pp. 227-237
Abstract: Aim of this study was to examine synaptic connectivity changes in the retina and the location and rate of apoptosis in transgenic S334ter line-3 and line-5 rats with photoreceptor degeneration. Heterozygous S334ter-line-3 and line-5 at P11-13, P30, P60, P90 and several control non-dystrophic rats (Long Evans and Sprague–Dawley) at P60, were studied anatomically by immunohistochemistry for various cell and synaptic markers, and by PNA and TUNEL label.- S334ter line-3 exhibited the fastest rate of degeneration with an early loss of photoreceptors, with 1–2 layers remaining at P30, and only cones left at P60. Line-5 had 4–5 layers left at P30, and very few rods left at P60-90. In both lines, horizontal cell processes (including dendrites and axon) were diminished at P11-13, showing gaps in the outer plexiform layer (OPL) at P60, and at P90, almost no terminal tips could be seen. Bipolar cells showed a retraction of their dendrites forming clusters along the OPL. Synaptic terminals of A-II amacrine cells in the IPL lost most of their parvalbumin-immunoreactivity. The apoptosis rate was different in both lines. Line-3 rats showed many photoreceptors affected at P11, occupying the innermost part of the outer nuclear layer. Line-5 showed a lower number of apoptotic cells within the same location at P13. In summary, the S334ter line-3 rat has a faster progression of degeneration than line-5. The horizontal and bipolar terminals are already affected at P11-P13 in both models. Apoptosis is related to the mutated rhodopsin transgene; the first photoreceptor cells affected are those close to the OPL.
Sponsor: This study was supported by Ministerio de Ciencia e Innovación BFU2009-07793/BFI, RETICS RD07/0062/0012, Fundaluce, ONCE, Fundación Médica Mutua Madrileña to NC Ministerio de Sanidad, FIS PS09/01854 to IP; and an anonymous sponsor, Foundation Fighting Blindness [MJS, RBA]; and NIH EY03040 [Doheny vivarium core grant].
ISSN: 0014-4835 (Print) | 1096-0007 (Online)
DOI: 10.1016/j.exer.2010.12.001
Language: eng
Type: info:eu-repo/semantics/article
Peer Review: si
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Appears in Collections:INV - NEUROVIS - Artículos de Revistas

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