Environmental control of phosphorylation pathways in a branched two-component system
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Título: | Environmental control of phosphorylation pathways in a branched two-component system |
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Autor/es: | López Redondo, María Luisa | Moronta Barrios, Félix | Salinas, Paloma | Espinosa, Javier | Cantos, Raquel | Dixon, Ray | Marina Moreno, Alberto | Contreras, Asunción |
Grupo/s de investigación o GITE: | Transducción de Señales en Bacterias |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología | Instituto de Biomedicina de Valencia | John Innes Centre. Department of Molecular Microbiology |
Palabras clave: | Two-component system | Cyanobacteria | Phosphorylation pathways |
Área/s de conocimiento: | Genética |
Fecha de creación: | 2010 |
Fecha de publicación: | 14-sep-2010 |
Editor: | Blackwell Publishing |
Cita bibliográfica: | LÓPEZ REDONDO, María Luisa, et al. "Environmental control of phosphorylation pathways in a branched two-component system". Molecular Microbiology. Vol. 78, Issue 2 (Oct. 2010). ISSN 0950-382X, pp. 475-489 |
Resumen: | NblS, the most conserved histidine kinase in cyanobacteria, regulates photosynthesis and acclimatization to a variety of environmental conditions. We used in silico, in vivo and in vitro approaches to identify RpaB and SrrA as the cognate response regulators of NblS and to characterize relevant interactions between components of this signalling system. While genetic analysis showed the importance of the NblS to RpaB phosphorylation branch for culture viability in Synechococcus elongatus PCC 7942, in vitro assays indicated a strong preference for NblS to phosphorylate SrrA. This apparent discrepancy can be explained by environmental insulation of the RpaB pathway, achieved by RpaB-dependent repression of srrA under standard, low light culture conditions. After a strong but transient increase in srrA expression upon high light exposure, negative regulation of srrA and other high light inducible genes takes place, suggesting cooperation between pathways under environmental conditions in which both RpaB and SrrA are present. Complex regulatory interactions between RpaB and SrrA, two response regulators with a common evolutionary origin that are controlled by a single histidine kinase, are thus emerging. Our results provide a paradigm for regulatory interactions between response regulators in a branched two-component system. |
Patrocinador/es: | This work was supported by Ministerio de Ciencia e Innovación (Grants BFU2009-07371, BIO2009-10872 and Prometeo/2009/051) and Ministerio de Educación (PR2009-0378). Thanks to Sociedad de Relaciones Internacionales, Universidad de Alicante, for Felix Moronta's grant. |
URI: | http://hdl.handle.net/10045/14961 |
ISSN: | 0950-382X (Print) | 1365-2958 (Online) |
DOI: | 10.1111/j.1365-2958.2010.07348.x |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | The definitive version is available at www.blackwell-synergy.com |
Revisión científica: | si |
Versión del editor: | http://dx.doi.org/10.1111/j.1365-2958.2010.07348.x |
Aparece en las colecciones: | INV - TSB - Artículos de Revistas |
Archivos en este ítem:
Archivo | Descripción | Tamaño | Formato | |
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Lopez-RedondoMolMicro2010.pdf | Articulo principal. Versión final (acceso restringido) | 1,22 MB | Adobe PDF | Abrir Solicitar una copia |
SI_Lopez-Redondo.pdf | Supporting information | 871,79 kB | Adobe PDF | Abrir Vista previa |
MolMicrobiol2010proof.pdf | Versión revisada (acceso libre) | 1,47 MB | Adobe PDF | Abrir Vista previa |
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