The localization of guanylyl cyclase-activating proteins in the mammalian retina

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Title: The localization of guanylyl cyclase-activating proteins in the mammalian retina
Authors: Cuenca, Nicolás | López Tomás, Salvador | Howes, Kim | Kolb, Helga
Research Group/s: Neurobiología del Sistema Visual y Terapia Génica de Enfermedades Neurodegenerativas
Center, Department or Service: Universidad de Alicante. Departamento de Biotecnología
Keywords: Guanylyl cylase-activating proteins | Mammalian retina
Knowledge Area: Oftalmología
Issue Date: Jun-1998
Publisher: Association for Research in Vision and Ophthalmology
Citation: CUENCA NAVARRO, Nicolás, et al. "The localization of guanylyl cyclase-activating proteins in the mammalian retina". Investigative Ophthalmology and Visual Science. Vol. 39, No. 7 (June 1998). ISSN 0146-0404, pp. 1243-1250
Abstract: PURPOSE: To explore the distribution of guanylyl cylase-activating proteins 1 and 2 (GCAP1 and GCAP2) in the mammalian retina. METHODS: Cryostat and vibratome vertical sections and wholemount retinas from mouse, rat, cat, bovine, monkey, and human eyes were prepared for immunocytochemistry and viewing by light and confocal microscopy. RESULTS: In all mammalian retinas investigated, intense GCAP1 immunoreactivity (GCAP1-IR) was seen in cone photoreceptor inner and outer segments, cell bodies, and synaptic regions. Intensity of the GCAP1-IR was strong in inner segments of rods in all species but weaker in outer segments-particularly so in primates and cats. GCAP2 immunoreactivity (GCAP2-IR) was weak in bovine, mouse, and rat cones but was intense in human and monkey cones. In all species except primates, GCAP2 staining was intense in rod inner and outer segments. In primates GCAP2-IR was intense in the rod inner segment but faint in the rod outer segment. A striking difference from the GCAP1 pattern of immunoreactivity was seen with GCAP2 antibodies as far as the inner retina was concerned. GCAP2-IR was evident in certain populations of bipolar, amacrine, and ganglion cells in all species. CONCLUSIONS: GCAP1 and GCAP2, which are involved in Ca2+-dependent stimulation and inhibition of photoreceptor guanylyl cyclase, can be detected in mammalian photoreceptor inner and outer segments, consistent with their physiological function. The occurrence of both GCAPs in the synaptic region of the photoreceptors indicates participation of these proteins in pathways other than regulation of phototransduction. The occurrence of GCAP2 in inner retinal neurons is indicative of second-messenger chemical transduction, possibly in metabotropic glutamate, gamma-aminobutyric acid (GABA) receptor, and nitric oxide-activated neural circuits.
Sponsor: Supported by grant EYO3323 from the National Eye Institute, National Institutes of Health, Bethesda, Maryland; a grant to the Department of Ophthalmology, Moran Eye Center, Salt Lake City, Utah; and a grant from Research to Prevent Blindness, New York, New York. NC was supported in part by grant DGICYT PB 94-1501 from the government of Valencia, Spain. The confocal microscope in the Moran Eye Center is supported by donations from the Lions Clubs of Utah.
ISSN: 0146-0404 (Print) | 1552-5783 (Online)
Language: eng
Type: info:eu-repo/semantics/article
Peer Review: si
Appears in Collections:INV - NEUROVIS - Artículos de Revistas

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