Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities

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Title: Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities
Authors: Salgado, Oscar | Guajardo-Leiva, Sergio | Moya-Beltrán, Ana | Barbosa, Carla | Ridley, Christina | Tamayo-Leiva, Javier | Quatrini, Raquel | Mojica, Francisco J.M. | Díez, Beatriz
Research Group/s: Microbiología Molecular
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Cas1 | Hot spring | Phylogenomic | CRISPR-Cas | Casposase
Issue Date: 2-Dec-2022
Publisher: Frontiers Media
Citation: Salgado O, Guajardo-Leiva S, Moya-Beltrán A, Barbosa C, Ridley C, Tamayo-Leiva J, Quatrini R, Mojica FJM and Díez B (2022) Global phylogenomic novelty of the Cas1 gene from hot spring microbial communities. Front. Microbiol. 13:1069452. doi: 10.3389/fmicb.2022.1069452
Abstract: The Cas1 protein is essential for the functioning of CRISPR-Cas adaptive systems. However, despite the high prevalence of CRISPR-Cas systems in thermophilic microorganisms, few studies have investigated the occurrence and diversity of Cas1 across hot spring microbial communities. Phylogenomic analysis of 2,150 Cas1 sequences recovered from 48 metagenomes representing hot springs (42–80°C, pH 6–9) from three continents, revealed similar ecological diversity of Cas1 and 16S rRNA associated with geographic location. Furthermore, phylogenetic analysis of the Cas1 sequences exposed a broad taxonomic distribution in thermophilic bacteria, with new clades of Cas1 homologs branching at the root of the tree or at the root of known clades harboring reference Cas1 types. Additionally, a new family of casposases was identified from hot springs, which further completes the evolutionary landscape of the Cas1 superfamily. This ecological study contributes new Cas1 sequences from known and novel locations worldwide, mainly focusing on under-sampled hot spring microbial mat taxa. Results herein show that circumneutral hot springs are environments harboring high diversity and novelty related to adaptive immunity systems.
Sponsor: This work was financed in part by FONDECYT regular N° 1190998 (ANID) and Iniciativa de Investigación UnACh 2021-157-Unach. OS and JT-L were supported in part by ANID National Doctoral Scholarship (Beca de Doctorado Nacional ANID) N° 21172022 and 21171048, respectively. SG-L was supported by ANID FONDECYT Postdoctoral N° 3210547. AM-B and RQ were supported by Centro Ciencia and Vida, FB210008, Financiamiento Basal para Centros Científicos y Tecnológicos de Excelencia de ANID, and FONDECYT regular N° 1221035 (ANID). FJMM acknowledged research support by the Conselleria d’Innovació, Universitats, Ciència i Societat Digital from Generalitat Valenciana, research project PROMETEO/2021/057. BD acknowledged the Millennium Institute Center for Genome Regulation, Project ICN2021-044 supported by the ANID Millennium Scientific Initiative (Chile).
ISSN: 1664-302X
DOI: 10.3389/fmicb.2022.1069452
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2022 Salgado, Guajardo-Leiva, Moya-Beltrán, Barbosa, Ridley, Tamayo-Leiva, Quatrini, Mojica and Díez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Peer Review: si
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Appears in Collections:INV - Microbiología Molecular - Artículos de Revistas

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