Neuroprotective Effects of Tauroursodeoxicholic Acid Involves Vascular and Glial Changes in Retinitis Pigmentosa Model

Empreu sempre aquest identificador per citar o enllaçar aquest ítem http://hdl.handle.net/10045/123145
Información del item - Informació de l'item - Item information
Títol: Neuroprotective Effects of Tauroursodeoxicholic Acid Involves Vascular and Glial Changes in Retinitis Pigmentosa Model
Autors: Fernández-Sánchez, Laura | Albertos Arranz, Henar | Ortuño-Lizarán, Isabel | Lax, Pedro | Cuenca, Nicolás
Grups d'investigació o GITE: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Centre, Departament o Servei: Universidad de Alicante. Departamento de Óptica, Farmacología y Anatomía | Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Paraules clau: Neurovascular unit | Astrogliosis | Neuroprotection | Vascular network | Neurodegenerative diseases | Retina | Bile acids
Àrees de coneixement: Farmacología | Biología Celular | Fisiología
Data de publicació: 12-d’abril-2022
Editor: Frontiers Media
Citació bibliogràfica: Fernández-Sánchez L, Albertos-Arranz H, Ortuño-Lizarán I, Lax P and Cuenca N (2022) Neuroprotective Effects of Tauroursodeoxicholic Acid Involves Vascular and Glial Changes in Retinitis Pigmentosa Model. Front. Neuroanat. 16:858073. doi: 10.3389/fnana.2022.858073
Resum: Purpose: Retinitis pigmentosa is primarily characterized by a massive photoreceptor loss. But a global retinal remodeling occurs in later stages of the disease. At that phase, glial cells and retinal vasculature are also strongly affected. The main aim of the present work is to assess if the bile acid Tauroursodeoxicholic acid (TUDCA), which has a demonstrated neuroprotective effect in numerous neurodegenerative diseases, is able to prevent glial and vascular degeneration in the P23H rat retina. Methods: Homozygous P23H (line 3) animals were injected weekly with a TUDCA (500 mg/kg, i.p.) or vehicle solution, from the postnatal day (P) 21 to P120. Sprague-Dawley rats (SD) were used as control. Retinal cross-sections and wholemounts were immunostained using different glial and vascular markers and visualized with confocal microscopy. Retinal blood vessels were stained with nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry and retinal vascular networks were drawn by hand using a camera lucida. Results: At P120, the photoreceptor degeneration observed in P23H rats was accompanied by a reduction in the vascular network density and complexity at the deep capillary plexus. In addition, astrocytes showed gliotic features and the outer processes of Müller cells displayed an aberrant distribution in ring-shaped structures. When treated with TUDCA, P23H rats displayed better-preserved vessels and capillary loops in the deep capillary plexus which are associated with the partial preservation of photoreceptors. TUDCA treatment also increased the number of astrocytes and reduced the presence of Müller cell process clusters in the outer retina. Conclusion: This work suggests that, besides its neuroprotective effect on photoreceptor cells, TUDCA treatment also protects from vascular and glial degeneration, a fact that encourages the use of TUDCA as a powerful therapy for neurodegenerative diseases.
Patrocinadors: The authors also acknowledge support from grants funded by the Spanish Ministry of Science and Innovation (FEDER-PID2019-106230RB-I00), Spanish Ministry of Universities (FPU18/02964), National Institute of Health Carlos III (RETICS-FEDER RD16/0008/0016), and Generalitat Valenciana (IDIFEDER/2017/064, PROMETEO/2021/024, GV/2020/028, and APOSTD/2020/245).
URI: http://hdl.handle.net/10045/123145
ISSN: 1662-5129
DOI: 10.3389/fnana.2022.858073
Idioma: eng
Tipus: info:eu-repo/semantics/article
Drets: © 2022 Fernández-Sánchez, Albertos-Arranz, Ortuño-Lizarán, Lax and Cuenca. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Revisió científica: si
Versió de l'editor: https://doi.org/10.3389/fnana.2022.858073
Apareix a la col·lecció: INV - NEUROVIS - Artículos de Revistas

Arxius per aquest ítem:
Arxius per aquest ítem:
Arxiu Descripció Tamany Format  
ThumbnailFernandez-Sanchez_etal_2022_FrontNeuroanat.pdf12,02 MBAdobe PDFObrir Vista prèvia


Aquest ítem està subjecte a una llicència de Creative Commons Llicència Creative Commons Creative Commons