Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa

Please use this identifier to cite or link to this item:
Información del item - Informació de l'item - Item information
Title: Decrease in DHA and other fatty acids correlates with photoreceptor degeneration in retinitis pigmentosa
Authors: Ruiz-Pastor, María José | Kutsyr, Oksana | Lax, Pedro | Cuenca, Nicolás
Research Group/s: Neurobiología del Sistema Visual y Terapia de Enfermedades Neurodegenerativas (NEUROVIS)
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Fatty acids | DHA | Omega-3 | Lipidomics | Retinitis pigmentosa | Cell death | Photoreceptors
Knowledge Area: Biología Celular | Fisiología
Issue Date: 10-Jun-2021
Publisher: Elsevier
Citation: Experimental Eye Research. 2021, 209: 108667.
Abstract: Fatty acids, and especially docosahexaenoic acid (DHA), are essential for photoreceptor cell integrity and are involved in the phototransduction cascade. In this study, we analyzed the changes in the fatty acid profile in the retina of the rd10 mouse model of retinitis pigmentosa in order to identify potential risk factors for retinal degeneration and possible therapeutic approaches. Fatty acids from C57BL/6J and rd10 mouse retinas were extracted with Folch's method and analyzed by gas chromatography/mass spectrometry. Changes in retinal morphology were evaluated by immunohistochemistry. The rd10 mouse retina showed a decreased number of photoreceptor rows and alterations in photoreceptor morphology compared to C57BL/6J mice. The total amount of fatty acids dropped by 29.4% in the dystrophic retinas compared to C57BL/6J retinas. A positive correlation was found between the retinal content of specific fatty acids and the number of photoreceptor rows. We found that the amount of several short-chain and long-chain saturated fatty acids, as well as monounsaturated fatty acids, decreased in the retina of rd10 mice. Moreover, the content of the n-6 polyunsaturated fatty acid arachidonic acid and the n-3 polyunsaturated DHA decreased markedly in the dystrophic retina. The fall of DHA was more pronounced, hence the n-6/n-3 ratio was significantly increased in the diseased retina. The content of specific fatty acids in the retina decreases with photoreceptor degeneration in retinitis pigmentosa mice, with a remarkable reduction in DHA and other saturated and unsaturated fatty acids. These fatty acids could be essential for photoreceptor cell viability, and they should be evaluated for the design of therapeutical strategies and nutritional supplements.
Sponsor: This work was supported by Spanish Ministry of Economy and Competitiveness [MICINN-FEDER PID 2019-106230RB-I00, 2020]; Instituto de Salud Carlos III [RETICS-FEDER RD16/0008/0016, 2016]; and Generalitat Valenciana [ACIF/2020/203, 2020], [IDIFEDER/2017/064, 2017].
ISSN: 0014-4835 (Print) | 1096-0007 (Online)
DOI: 10.1016/j.exer.2021.108667
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (
Peer Review: si
Publisher version:
Appears in Collections:INV - NEUROVIS - Artículos de Revistas

Files in This Item:
Files in This Item:
File Description SizeFormat 
ThumbnailRuiz-Pastor_etal_2021_ExperimEyeRes.pdf6,22 MBAdobe PDFOpen Preview

Items in RUA are protected by copyright, with all rights reserved, unless otherwise indicated.