Chitosan inhibits septin‐mediated plant infection by the rice blast fungus Magnaportheoryzae in a protein kinase C and Nox1 NADPH oxidase‐dependent manner
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Título: | Chitosan inhibits septin‐mediated plant infection by the rice blast fungus Magnaportheoryzae in a protein kinase C and Nox1 NADPH oxidase‐dependent manner |
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Autor/es: | Lopez-Moya, Federico | Martin-Urdiroz, Magdalena | Oses-Ruiz, Miriam | Were, Vincent M. | Fricker, Mark D. | Littlejohn, George | Lopez-Llorca, Luis Vicente | Talbot, Nicholas J. |
Grupo/s de investigación o GITE: | Fitopatología |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Ciencias del Mar y Biología Aplicada |
Palabras clave: | Actin | Chitosan | Magnaporthe oryzae | Membrane permeabilization | NADPH oxidase | Pkc1 pathway | Reactive oxygen species (ROS) | Septin |
Área/s de conocimiento: | Botánica |
Fecha de publicación: | may-2021 |
Editor: | Wiley | New Phytologist Foundation |
Cita bibliográfica: | New Phytologist. 2021, 230(4): 1578-1593. https://doi.org/10.1111/nph.17268 |
Resumen: | Chitosan is a partially deacetylated linear polysaccharide composed of β‐1,4‐linked units of d‐glucosamine and N‐acetyl glucosamine. As well as a structural component of fungal cell walls, chitosan is a potent antifungal agent. However, the mode of action of chitosan is poorly understood. Here, we report that chitosan is effective for control of rice blast disease. Chitosan application impairs growth of the blast fungus Magnaporthe oryzae and has a pronounced effect on appressorium‐mediated plant infection. Chitosan inhibits septin‐mediated F‐actin remodelling at the appressorium pore, thereby preventing repolarization of the infection cell. Chitosan causes plasma membrane permeabilization of M. oryzae and affects NADPH oxidase‐dependent synthesis of reactive oxygen species, essential for septin ring formation and fungal pathogenicity. We further show that toxicity of chitosan to M. oryzae requires the protein kinase C‐dependent cell wall integrity pathway, the Mps1 mitogen‐activated protein kinase and the Nox1 NADPH oxidase. A conditionally lethal, analogue (PP1)‐sensitive mutant of Pkc1 is partially remediated for growth in the presence of chitosan, while ∆nox1 mutants increase their glucan : chitin cell wall ratio, rendering them resistant to chitosan. Taken together, our data show that chitosan is a potent fungicide which requires the cell integrity pathway, disrupts plasma membrane function and inhibits septin‐mediated plant infection. |
Patrocinador/es: | This work was supported by AGL 2015 66833-R grant from the Spanish Ministry of Economy and Competitiveness and European H2020 Project MUSA-727624 and an EMBO Short-term Fellowship to FL-M. Work in NJT’s laboratory is supported by the Gatsby Charitable Foundation. |
URI: | http://hdl.handle.net/10045/114344 |
ISSN: | 0028-646X (Print) | 1469-8137 (Online) |
DOI: | 10.1111/nph.17268 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2021 The Authors New Phytologist © 2021 New Phytologist Foundation. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.1111/nph.17268 |
Aparece en las colecciones: | Investigaciones financiadas por la UE INV - Fitopatología - Artículos de Revistas |
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