Antibacterial activity testing methods for hydrophobic patterned surfaces

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Title: Antibacterial activity testing methods for hydrophobic patterned surfaces
Authors: Perez-Gavilan, Ana | Vieira de Castro, Joana | Arana, Ainara | Merino, Santos | Retolaza, Aritz | Alves, Sofia A. | Francone, Achille | Kehagias, Nikolaos | Sotomayor-Torres, Clivia M. | Cocina, Donato | Mortera, Renato | Crapanzano, Salvatore | Pelegrín, Carlos Javier | Garrigós, María del Carmen | Jiménez, Alfonso | Galindo, Begoña | Araque, Mari Carmen | Dykeman, Donna | Neves, Nuno M. | Marimón, Jose Maria
Research Group/s: Análisis de Polímeros y Nanomateriales
Center, Department or Service: Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología
Keywords: Antibacterial activity | Testing methods | Hydrophobic patterned surfaces
Knowledge Area: Química Analítica
Issue Date: 23-Mar-2021
Publisher: Springer Nature
Citation: Scientific Reports. 2021, 11: 6675.
Abstract: One strategy to decrease the incidence of hospital-acquired infections is to avoid the survival of pathogens in the environment by the development of surfaces with antimicrobial activity. To study the antibacterial behaviour of active surfaces, different approaches have been developed of which ISO 22916 is the standard. To assess the performance of different testing methodologies to analyse the antibacterial activity of hydrophobic surface patterned plastics as part of a Horizon 2020 European research project. Four different testing methods were used to study the antibacterial activity of a patterned film, including the ISO 22916 standard, the immersion method, the touch-transfer inoculation method, and the swab inoculation method, this latter developed specifically for this project. The non-realistic test conditions of the ISO 22916 standard showed this method to be non-appropriate in the study of hydrophobic patterned surfaces. The immersion method also showed no differences between patterned films and smooth controls due to the lack of attachment of testing bacteria on both surfaces. The antibacterial activity of films could be demonstrated by the touch-transfer and the swab inoculation methods, that more precisely mimicked the way of high-touch surfaces contamination, and showed to be the best methodologies to test the antibacterial activity of patterned hydrophobic surfaces. A new ISO standard would be desirable as the reference method to study the antibacterial behaviour of patterned surfaces.
Sponsor: The present study was carried out as part of a European project called “Antimicrobial FLEXible POLymers for its use in hospital environments” (FLEXPOL Grant agreement No. 721062) funded by Horizon 2020 Framework Programme for Research and Innovation (2014-2020).
ISSN: 2045-2322
DOI: 10.1038/s41598-021-85995-9
Language: eng
Type: info:eu-repo/semantics/article
Rights: © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit
Peer Review: si
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Appears in Collections:Research funded by the EU
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