Haloferax mediterranei, an Archaeal Model for Denitrification in Saline Systems, Characterized Through Integrated Physiological and Transcriptional Analyses
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Título: | Haloferax mediterranei, an Archaeal Model for Denitrification in Saline Systems, Characterized Through Integrated Physiological and Transcriptional Analyses |
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Autor/es: | Torregrosa-Crespo, Javier | Pire, Carmen | Bergaust, Linda | Martínez-Espinosa, Rosa María |
Grupo/s de investigación o GITE: | Bioquímica Aplicada/Applied Biochemistry (AppBiochem) |
Centro, Departamento o Servicio: | Universidad de Alicante. Departamento de Agroquímica y Bioquímica |
Palabras clave: | Haloferax | Denitrification | Nitrogenous gases | Nitrate reductase | Nitrite reductase | Extremophile |
Área/s de conocimiento: | Bioquímica y Biología Molecular |
Fecha de publicación: | 22-abr-2020 |
Editor: | Frontiers Media |
Cita bibliográfica: | Torregrosa-Crespo J, Pire C, Bergaust L and Martínez-Espinosa RM (2020) Haloferax mediterranei, an Archaeal Model for Denitrification in Saline Systems, Characterized Through Integrated Physiological and Transcriptional Analyses. Front. Microbiol. 11:768. doi: 10.3389/fmicb.2020.00768 |
Resumen: | Haloferax mediterranei (R4) belongs to the group of halophilic archaea, one of the predominant microbial populations in hypersaline environments. In these ecosystems, the low availability of oxygen pushes the microbial inhabitants toward anaerobic pathways and the presence of N-oxyanions favor denitrification. In a recent study comparing three Haloferax species carrying dissimilatory N-oxide reductases, H. mediterranei showed promise as a future model for archaeal denitrification. This work further explores the respiratory physiology of this haloarchaeon when challenged with ranges of nitrite and nitrate concentrations and at neutral or sub-neutral pH during the transition to anoxia. Moreover, to begin to understand the transcriptional regulation of N-oxide reductases, detailed gas kinetics was combined with gene expression analyses at high resolution. The results show that H. mediterranei has an expression pattern similar to that observed in the bacterial Domain, well-coordinated at low concentrations of N-oxyanions. However, it could only sustain a few generations of exponential anaerobic growth, apparently requiring micro-oxic conditions for de novo synthesis of denitrification enzymes. This is the first integrated study within this field of knowledge in haloarchaea and Archaea in general, and it sheds lights on denitrification in salty environments. |
Patrocinador/es: | This work was funded by research grants from the MINECO Spain (CTM2013-43147R and RTI2018-099860-B-I00), VIGROB-309 (University of Alicante), and Generalitat Valenciana (ACIF 2016/077). JT-C was financed by the European Molecular Biology Organization (EMBO—Short Term Fellowship ASTF No: 331-2016) to carry out the experiments at the Norwegian University of Life Sciences (NMBU). LB was funded by The Research Council of Norway (Project No. 275389). |
URI: | http://hdl.handle.net/10045/105987 |
ISSN: | 1664-302X |
DOI: | 10.3389/fmicb.2020.00768 |
Idioma: | eng |
Tipo: | info:eu-repo/semantics/article |
Derechos: | © 2020 Torregrosa-Crespo, Pire, Bergaust and Martínez-Espinosa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Revisión científica: | si |
Versión del editor: | https://doi.org/10.3389/fmicb.2020.00768 |
Aparece en las colecciones: | INV - AppBiochem - Artículos de Revistas |
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