Cuenca, Nicolás, Fernández-Sánchez, Laura, Sauvé, Yves, Segura, Francisco, Martínez Navarrete, Gema Concepción, Tamarit, José Manuel, Fuentes-Broto, Lorena, Sánchez Cano, Ana, Pinilla Lozano, Isabel
Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration
Cuenca N, Fernández-Sánchez L, Sauvé Y, Segura FJ, Martínez-Navarrete G, Tamarit JM, Fuentes-Broto L, Sanchez-Cano A and Pinilla I (2014) Correlation between SD-OCT, immunocytochemistry and functional findings in an animal model of retinal degeneration. Front. Neuroanat. 8:151. doi: 10.3389/fnana.2014.00151
URI: http://hdl.handle.net/10045/54650
DOI: 10.3389/fnana.2014.00151
ISSN: 1662-5129
Abstract: 
Purpose: The P23H rhodopsin mutation is an autosomal dominant cause of retinitis pigmentosa (RP). The degeneration can be tracked using different anatomical and functional methods. In our case, we evaluated the anatomical changes using Spectral-Domain Optical Coherence Tomography (SD-OCT) and correlated the findings with retinal thickness values determined by immunocytochemistry.Methods: Pigmented rats heterozygous for the P23H mutation, with ages between P18 and P180 were studied. Function was assessed by means of optomotor testing and ERGs. Retinal thicknesses measurements, autofluorescence and fluorescein angiography were performed using Spectralis OCT. Retinas were studied by means of immunohistochemistry. Results: Between P30 and P180, visual acuity decreased from 0.500 to 0.182 cycles per degree (cyc/deg) and contrast sensitivity decreased from 54.56 to 2.98 for a spatial frequency of 0.089 cyc/deg. Only cone-driven b-wave responses reached developmental maturity. Flicker fusions were also comparable at P29 (42 Hz). Double flash-isolated rod-driven responses were already affected at P29. Photopic responses revealed deterioration after P29.A reduction in retinal thicknesses and morphological modifications were seen in OCT sections. Statistically significant differences were found in all evaluated thicknesses. Autofluorescence was seen in P23H rats as sparse dots. Immunocytochemistry showed a progressive decrease in the outer nuclear layer (ONL), and morphological changes. Although anatomical thickness measures were significantly lower than OCT values, there was a very strong correlation between the values measured by both techniques.Conclusions: In pigmented P23H rats, a progressive deterioration occurs in both retinal function and anatomy. Anatomical changes can be effectively evaluated using SD-OCT and immunocytochemistry, with a good correlation between their values, thus making SD-OCT an important tool for research in retinal degeneration.
Keywords:Retinal degeneration, Retinitis pigmentosa, P23H rat, Visual acuity, Electroretinogram, Optical coherence tomography, Autofluorescence, Immunohistochemistry
Frontiers Media
info:eu-repo/semantics/article