Focused Metabolism of β-Glucans by the Soil Bacteroidetes Species Chitinophaga pinensis

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Title: Focused Metabolism of β-Glucans by the Soil Bacteroidetes Species Chitinophaga pinensis
Authors: McKee, Lauren S. | Martinez-Abad, Antonio | Ruthes, Andrea C. | Vilaplana, Francisco | Brumer, Harry
Research Group/s: Análisis de Polímeros y Nanomateriales
Center, Department or Service: Universidad de Alicante. Departamento de Química Analítica, Nutrición y Bromatología
Keywords: β-glucan polysaccharides | Bacteria | Bacteroidetes | Biomass recycling | Carbohydrate active enzymes | Polysaccharide utilization loci
Knowledge Area: Química Analítica
Issue Date: 9-Jan-2019
Publisher: American Society for Microbiology
Citation: Applied and Environmental Microbiology. 2019, 85(2): e02231-18. doi:10.1128/AEM.02231-18
Abstract: The genome and natural habitat of Chitinophaga pinensis suggest it has the ability to degrade a wide variety of carbohydrate-based biomass. Complementing our earlier investigations into the hydrolysis of some plant polysaccharides, we now show that C. pinensis can grow directly on spruce wood and on the fungal fruiting body. Growth was stronger on fungal material, although secreted enzyme activity was high in both cases, and all biomass-induced secretomes showed a predominance of β-glucanase activities. We therefore conducted a screen for growth on and hydrolysis of β-glucans isolated from different sources. Most noncrystalline β-glucans supported good growth, with variable efficiencies of polysaccharide deconstruction and oligosaccharide uptake, depending on the polysaccharide backbone linkage. In all cases, β-glucan was the only type of polysaccharide that was effectively hydrolyzed by secreted enzymes. This contrasts with the secretion of enzymes with a broad range of activities observed during growth on complex heteroglycans. Our findings imply a role for C. pinensis in the turnover of multiple types of biomass and suggest that the species may have two metabolic modes: a “scavenging mode,” where multiple different types of glycan may be degraded, and a more “focused mode” of β-glucan metabolism. The significant accumulation of some types of β-gluco-oligosaccharides in growth media may be due to the lack of an appropriate transport mechanism, and we propose that this is due to the specificity of expressed polysaccharide utilization loci. We present a hypothetical model for β-glucan metabolism by C. pinensis that suggests the potential for nutrient sharing among the microbial litter community.
Sponsor: This work was supported by funds from the Knut and Alice Wallenberg foundation via the Wallenberg Wood Science Centre (to L.S.M.) and the Swedish Research Council Vetenskapsrådet (project 621-2014-5295 to F.V. and project 2017-04906 to L.S.M.).
URI: http://hdl.handle.net/10045/86874
ISSN: 0099-2240 (Print) | 1098-5336 (Online)
DOI: 10.1128/AEM.02231-18
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2019 American Society for Microbiology
Peer Review: si
Publisher version: https://doi.org/10.1128/AEM.02231-18
Appears in Collections:INV - NANOBIOPOL - Artículos de Revistas

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