Denitrifying haloarchaea within the genus Haloferax display divergent respiratory phenotypes, with implications for their release of nitrogenous gases

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Title: Denitrifying haloarchaea within the genus Haloferax display divergent respiratory phenotypes, with implications for their release of nitrogenous gases
Authors: Torregrosa-Crespo, Javier | Pire, Carmen | Martínez-Espinosa, Rosa María | Bergaust, Linda
Research Group/s: Bioquímica Aplicada/Applied Biochemistry (AppBiochem)
Center, Department or Service: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Keywords: Haloarchaea | Denitrification | Haloferax | Respiratory phenotypes | Nitrogenous gases
Knowledge Area: Bioquímica y Biología Molecular
Issue Date: 12-Nov-2018
Publisher: Wiley
Citation: Environmental Microbiology. 2019, 21(1): 427-436. doi:10.1111/1462-2920.14474
Abstract: Haloarchaea are extremophiles, generally thriving at high temperatures and salt concentrations, thus with limited access to oxygen. As a strategy to maintain a respiratory metabolism, many halophilic archaea are capable of denitrification. Among them are members of the genus Haloferax, which are abundant in saline/hypersaline environments. Three reported haloarchaeal denitrifiers, Haloferax mediterranei, Haloferax denitrificans and Haloferax volcanii, were characterized with respect to their denitrification phenotype. A semi‐automatic incubation system was used to monitor the depletion of electron acceptors and accumulation of gaseous intermediates in batch cultures under a range of conditions. Out of the species tested, only H. mediterranei was able to consistently reduce all available N‐oxyanions to N2, whilst the other two released significant amounts of NO and N2O, which affect tropospheric and stratospheric chemistries, respectively. The prevalence and magnitude of hypersaline ecosystems are on the rise due to climate change and anthropogenic activity. Thus, the biology of halophilic denitrifiers is inherently interesting, due to their contribution to the global nitrogen cycle, and potential application in bioremediation. This work is the first detailed physiological study of denitrification in haloarchaea, and as such a seed for our understanding of the drivers of nitrogen turnover in hypersaline systems.
Sponsor: This work was funded by research grant from the MINECO Spain (CTM2013-43147-R) and Generalitat Valenciana (ACIF 2016/077). L. Bergaust was financed by the Norwegian Research Council through FRIMEDIO (Grants 231282 and 275389).
URI: http://hdl.handle.net/10045/83647
ISSN: 1462-2912 (Print) | 1462-2920 (Online)
DOI: 10.1111/1462-2920.14474
Language: eng
Type: info:eu-repo/semantics/article
Rights: © Society for Applied Microbiology and John Wiley & Sons Ltd
Peer Review: si
Publisher version: https://doi.org/10.1111/1462-2920.14474
Appears in Collections:INV - AppBiochem - Artículos de Revistas

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