The effect of aerobic and anaerobic training with melatonin consumption on the expression of apoptotic genes BAX and BCL2 myocardial in rats after ischemic reperfusion

Abstract

The present research aims to examine The Effect of aerobic and anaerobic training with melatonin consumption on the expression of apoptotic genes BAX and BCL2 myocardial in rats after ischemic reperfusion. In this study, Wistar rats (n = 38) were used weighing approximately 200-250 g with two to three months old. The study was conducted using Stroke (n = 7) and healthy (n = 7) rats to determine the stroke of isoprenaline injection; and left ventricular stroke was confirmed after the isoprenaline injection with the Terry Chromasone technique. Then, the rats were divided into 6 groups of aerobic (n = 4), anaerobic (n = 4), aerobic with melatonin (n = 4), anaerobic with melatonin (n = 4), Melatonin (n = 4) and control group (n = 4). The research group rats were familiar running on treadmill for a week, and One-month aerobic and anaerobic training was performed with three sessions per week. Also, melatonin gavage (10 mg / kg) was applied to melatonin treatment groups. After two rest days, all rats were injected with isoprenaline at 24 hours intervals. However, control group rats were only injected with isoprenaline. Data was analyzed using independent T, one way ANOVA and 2-ΔΔct formula. The results of this study showed that aerobic and anaerobic training and melatonin alone had a negligible effect on BAX and BCL2 genes expression, However, aerobic and anaerobic training combined with melatonin was able to reduce and increase the expression of the BAX and BCL2 genes, respectively. Of course, these changes had a significant effect on melatonin alone. Therefore, it seems that this intensity and duration of training with melatonin intake into melatonin alone can provide the anti-apoptotic routes of training to minimize the effects of acute stroke.