Diversity of CRISPR loci in Escherichia coli

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Title: Diversity of CRISPR loci in Escherichia coli
Authors: Díez-Villaseñor, César | Almendros, Cristóbal | García-Martínez, Jesús | Mojica, Francisco J.M.
Research Group/s: Microbiología Molecular
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: MLEE | Multilocus enzyme electrophoresis | ECOR | Escherichia coli reference | CRISPR | Clustered regularly interspaced short palindromic repeats | CAS | CRISPR-associated sequence
Knowledge Area: Microbiología
Issue Date: 1-May-2010
Publisher: Microbiology Society
Citation: Microbiology. 2010, 156: 1351-1361. doi:10.1099/mic.0.036046-0
Abstract: CRISPR (clustered regularly interspaced short palindromic repeats) and CAS (CRISPR-associated sequence) proteins are constituents of a novel genetic barrier that limits horizontal gene transfer in prokaryotes by means of an uncharacterized mechanism. The fundamental discovery of small RNAs as the guides of the defence apparatus arose as a result of Escherichia coli studies. However, a survey of the system diversity in this species in order to further contribute to the understanding of the CRISPR mode of action has not yet been performed. Here we describe two CRISPR/CAS systems found in E. coli, following the analysis of 100 strains representative of the species' diversity. Our results substantiate different levels of activity between loci of both CRISPR types, as well as different target preferences and CRISPR relevances for particular groups of strains. Interestingly, the data suggest that the degeneration of one CRISPR/CAS system in E. coli ancestors could have been brought about by self-interference.
Sponsor: This work was financed by research grants from the Conselleria de Cultura, Educació i Ciència, Generalitat Valenciana (CTIDIB/2002/155) and the Ministerio de Educación y Ciencia (BIO2004-00523).
URI: http://hdl.handle.net/10045/75217
ISSN: 1350-0872 (Print) | 1465-2080 (Online)
DOI: 10.1099/mic.0.036046-0
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2010 SGM
Peer Review: si
Publisher version: https://doi.org/10.1099/mic.0.036046-0
Appears in Collections:INV - Microbiología Molecular - Artículos de Revistas

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