Removal of the blue component of light significantly decreases retinal damage after high intensity exposure

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Title: Removal of the blue component of light significantly decreases retinal damage after high intensity exposure
Authors: Vicente-Tejedor, Javier | Marchena, Miguel | Ramírez, Laura | García Ayuso, Diego | Gómez-Vicente, Violeta | Sánchez-Ramos, Celia | Villa, Pedro de la | Germain Martínez, Francisco
Research Group/s: Grupo de Óptica y Percepción Visual (GOPV)
Center, Department or Service: Universidad de Alicante. Departamento de Óptica, Farmacología y Anatomía
Keywords: Light damage | Photoreceptor | Electroretinogram | Short wavelength filter | Blue light
Knowledge Area: Óptica
Issue Date: 15-Mar-2018
Publisher: Public Library of Science (PLoS)
Citation: Vicente-Tejedor J, Marchena M, Ramírez L, García-Ayuso D, Gómez-Vicente V, Sánchez-Ramos C, et al. (2018) Removal of the blue component of light significantly decreases retinal damage after high intensity exposure. PLoS ONE 13(3): e0194218. https://doi.org/10.1371/journal.pone.0194218
Abstract: Light causes damage to the retina (phototoxicity) and decreases photoreceptor responses to light. The most harmful component of visible light is the blue wavelength (400–500 nm). Different filters have been tested, but so far all of them allow passing a lot of this wavelength (70%). The aim of this work has been to prove that a filter that removes 94% of the blue component may protect the function and morphology of the retina significantly. Three experimental groups were designed. The first group was unexposed to light, the second one was exposed and the third one was exposed and protected by a blue-blocking filter. Light damage was induced in young albino mice (p30) by exposing them to white light of high intensity (5,000 lux) continuously for 7 days. Short wavelength light filters were used for light protection. The blue component was removed (94%) from the light source by our filter. Electroretinographical recordings were performed before and after light damage. Changes in retinal structure were studied using immunohistochemistry, and TUNEL labeling. Also, cells in the outer nuclear layer were counted and compared among the three different groups. Functional visual responses were significantly more conserved in protected animals (with the blue-blocking filter) than in unprotected animals. Also, retinal structure was better kept and photoreceptor survival was greater in protected animals, these differences were significant in central areas of the retina. Still, functional and morphological responses were significantly lower in protected than in unexposed groups. In conclusion, this blue-blocking filter decreases significantly photoreceptor damage after exposure to high intensity light. Actually, our eyes are exposed for a very long time to high levels of blue light (screens, artificial light LED, neons…). The potential damage caused by blue light can be palliated.
Sponsor: This study was supported by project grants #SAF2010-21879 from Ministerio de Ciencia e Innovación (http://www.idi.mineco.gob.es), FIS #PI13-02098 and RETICS #RD12-0034-0006 from Instituto de Salud Carlos III (http://www.isciii.es) to PdV; #BES-2005-8339 from Ministerio de Educación y Ciencia to JV-T; #PTQ-10-03588 from Ministerio de Ciencia e Innovación (http://www.idi.mineco.gob.es) to CS-R.
URI: http://hdl.handle.net/10045/74367
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0194218
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2018 Vicente-Tejedor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Peer Review: si
Publisher version: https://doi.org/10.1371/journal.pone.0194218
Appears in Collections:INV - GOPV - Artículos de Revistas

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