Outcomes of ureteroscopy miniaturization on tissue damage and tissue hypoxia in a pig model

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Title: Outcomes of ureteroscopy miniaturization on tissue damage and tissue hypoxia in a pig model
Authors: Caballero-Romeu, Juan-Pablo | Galán-Llopis, Juan-Antonio | Soria, Federico | Morcillo-Martín, Esther | Caballero, Pablo | De La Cruz-Conty, Julia E. | Romero-Maroto, Jesús
Research Group/s: Grupo Balmis de Investigación en Salud Comunitaria e Historia de la Ciencia
Center, Department or Service: Universidad de Alicante. Departamento de Enfermería Comunitaria, Medicina Preventiva y Salud Pública e Historia de la Ciencia
Keywords: Ureteroscopy miniaturization | Tissue damage | Tissue hypoxia
Knowledge Area: Enfermería
Issue Date: 11-Jan-2018
Publisher: Springer Nature
Citation: Scientific Reports. 2018, 8: 431. doi:10.1038/s41598-017-18885-8
Abstract: Miniaturization of ureteroscopy materials is intended to decrease tissue damage. However, tissue hypoxia and the gross and microscopic effects on tissue have not been adequately assessed. We compared the gross and microscopic effects of micro-ureteroscopy (m-URS) and conventional ureteroscopy (URS) on the urinary tract. We employed 14 pigs of the Large White race. URS was performed in one of the ureters with an 8/9.8 F ureteroscope, while a 4.85 F m-URS sheath was used in the contralateral ureter. Gross assessment of ureteral wall damage and ureteral orifice damage was performed. For microscopic assessment hematoxylin-eosin staining and immunohistochemistry for detection of tissue hypoxia were conducted. Regarding the macroscopic assessment of ureteral damage, substantial and significant differences were recorded using URS (C = 0.8), but not with m-URS. Microscopic assessment after staining with hematoxylin-eosin revealed greater epithelial desquamation in the URS group (p < 0.05). Pimonidazole staining revealed greater hypoxia in the epithelial cells than in the remainder of the ureteral layers. We conclude that m-URS causes less damage to the ureteral orifice than URS. Histopathological findings show m-URS reduces ureteral epithelial damage compared with conventional ureteroscopy. Both URS and m-URS cause cellular hypoxia.
Sponsor: The present study is funded by the Alicante Institute for Health and Biomedical Research (ISABIAL-FISABIO Foundation) and Presurgy S.L.
URI: http://hdl.handle.net/10045/72539
ISSN: 2045-2322
DOI: 10.1038/s41598-017-18885-8
Language: eng
Type: info:eu-repo/semantics/article
Rights: Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Peer Review: si
Publisher version: http://dx.doi.org/10.1038/s41598-017-18885-8
Appears in Collections:INV - BISCHA - Artículos de Revistas

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