Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections

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Title: Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections
Authors: Galiana, Antonio | Coy, Javier | Gimeno, Adelina | Guzmán, Noemí M. | Rosales, Francisco | Merino, Esperanza | Royo, Gloria | Rodríguez, Juan Carlos
Research Group/s: Microbiología Molecular
Center, Department or Service: Universidad de Alicante. Departamento de Fisiología, Genética y Microbiología
Keywords: Blood infections | Diagnosis | Clinical microbiological methods | Sepsis Flow Chip assay
Knowledge Area: Microbiología
Issue Date: 18-May-2017
Publisher: Public Library of Science (PLoS)
Citation: Galiana A, Coy J, Gimeno A, Guzman NM, Rosales F, Merino E, et al. (2017) Evaluation of the Sepsis Flow Chip assay for the diagnosis of blood infections. PLoS ONE 12(5): e0177627. https://doi.org/10.1371/journal.pone.0177627
Abstract: Background: Blood infections are serious complex conditions that generally require rapid diagnosis and treatment. The big challenge is to reduce the time necessary to make a diagnosis with current clinical microbiological methods so as to improve the treatment given to patients. Methods: In this study, we assess for the first time the Sepsis Flow Chip assay, which is a novel diagnostic assay for simultaneous rapid-detection of the vast majority of bloodstream pathogens, including Gram-positive and Gram-negative bacteria and fungi, in the same assay, and for the detection of most common antibiotic resistance genes. The SFC assay is based on multiplex PCR and low density DNA arrays. Results: Positive blood cultures from 202 consecutive bacteremia patients were analyzed by SFC assay and the results were compared with the results obtained by the gold standard methodology used in clinical microbiology diagnostic laboratories (EUCAST guidelines). SFC assay overall sensitivity and specificity for bacterial identification were 93.3% and 100% respectively and sensitivity and specificity for the identification of antibiotic genetic resistance determinants were 93.6% and 100% respectively. Conclusions: This is the first evaluation of SFC assay in clinical samples. This new method appears to be very promising by combining the high number of distinct pathogens and genetic resistance determinants identified in a single assay. Further investigations should be done to evaluate the usefulness of this assay in combination with clinical multidisciplinary groups (stewardship), in order for the results to be applied appropriately to the management of patients ‘infectious processes.
Sponsor: This work was supported by Hospital General Universitario de Alicante (UGP-14-270) http://alicante.san.gva.es/; Fundación Soria Melguizo (no number) http://www.f-soria.es/; and FISABIO (UGP-14-216) http://fisabio.san.gva.es/.
URI: http://hdl.handle.net/10045/71835
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0177627
Language: eng
Type: info:eu-repo/semantics/article
Rights: © 2017 Galiana et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Peer Review: si
Publisher version: http://dx.doi.org/10.1371/journal.pone.0177627
Appears in Collections:INV - Microbiología Molecular - Artículos de Revistas

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