Effectiveness of human spermatozoa biomarkers as indicators of structural damage during cryopreservation

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Título: Effectiveness of human spermatozoa biomarkers as indicators of structural damage during cryopreservation
Autor/es: Gómez-Torres, María José | Medrano, María Llanos | Romero, Alejandro | Fernández Colom, Pedro José | Aizpurua, Jon
Grupo/s de investigación o GITE: Grupo de Inmunología, Biología Celular y del Desarrollo | Biotecnología
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Biotecnología
Palabras clave: Cryopreservation | Spermatozoa | Acrosome | DNA fragmentation | Cytoskeleton | Morphology | Cryodamage
Área/s de conocimiento: Biología Celular
Fecha de publicación: oct-2017
Editor: Elsevier
Cita bibliográfica: Cryobiology. 2017, 78: 90-94. doi:10.1016/j.cryobiol.2017.06.008
Resumen: Human spermatozoa cryopreservation techniques are used to maintain and protect male fertility in cases such as infertility and malignancy treatments. However, during cryopreservation, the spermatozoa's metabolic rate is reduced and they undergo dramatic functional and structural changes owing to exposure to cryoprotectants and freezing-thawing procedures. While the effects of cryopreservation on cells are documented, to date the induced cryodamage on structural and/or functional sperm biomarkers is not well established at multivariate scale. To address this question, we performed basic sperm analysis, sperm DNA fragmentation assessment, spontaneous acrosome reaction measurement, and cytoskeleton evaluation after thawing samples from subjects with normal and low-quality semen. A cryodamage rate was used to determine the effects of the freeze-thaw process on spermatozoa. In addition, a Principal Component Analysis (PCA) was used for data reduction and to evaluate sperm-specific patterns during the cryopreservation process. We found that the vitality, progressive motility and sperm count from low-quality samples after cryopreservation show higher damage rates (≥40%) than in normal sperm samples. However, cytoskeleton, DNA, tail and mid-piece and acrosome display the highest cryodamage rates (∼50–99%) and are equally susceptible to cryopreservation-induced damage in both low- and normal-quality semen samples. Overall, the evaluation of these parameters provides meaningful information about different aspects of sperm functionality after cryopreservation.
Patrocinador/es: We would like to appreciate financial support provided by the Cátedra Human Fertility (Universidad de Alicante, Alicante, Spain) grant number 60931013.
URI: http://hdl.handle.net/10045/69254
ISSN: 0011-2240 (Print) | 1090-2392 (Online)
DOI: 10.1016/j.cryobiol.2017.06.008
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2017 Elsevier Inc.
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1016/j.cryobiol.2017.06.008
Aparece en las colecciones:INV - Grupo de Inmunología - Artículos de Revistas
INV - GIDBT - Artículos de Revistas

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