Ultrastructural characteristics of human oocytes vitrified before and after in vitro maturation

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Title: Ultrastructural characteristics of human oocytes vitrified before and after in vitro maturation
Authors: Segovia Huertas, Yolanda | Victory, Noemí | Peinado, Irene | García-Valverde, Laura M. | García Irles, Magdalena | Aizpurua, Jon | Monzó, Ana | Gómez-Torres, María José
Research Group/s: Grupo de Inmunología, Biología Celular y del Desarrollo
Center, Department or Service: Universidad de Alicante. Departamento de Biotecnología
Keywords: Cryopreservation | Electron microscopy | In vitro maturation
Knowledge Area: Biología Celular
Issue Date: 30-Apr-2017
Publisher: Society for Reproduction and Development
Citation: Journal of Reproduction and Development. 2017, 63(4): 377-382. doi:10.1262/jrd.2017-009
Abstract: The development of an effective program that combines in vitro maturation (IVM) and cryopreservation for immature oocytes would represent a novel advance for in vitro fertilization (IVF), especially as a means to preserve the fertility of women in unique situations. The aim of this study was to analyze the ultrastructural characteristics of human oocytes, obtained after controlled ovarian stimulation, to determine whether IVM is best performed before or after vitrification. To this end, we analyzed the following features in a total of 22 MII oocytes: size, zona pellucida and perivitelline space, mitochondria number, M-SER (mitochondria-smooth endoplasmic reticulum) aggregates and M-V (mitochondria-vesicle) complexes, the number of cortical granules and microvilli, and the presence of vacuolization using transmission electron microscopy (TEM). Each oocyte presented a rounded shape, with an intact oolemma, and was surrounded by a continuous zona pellucida and perivitelline space. Statistical analysis comparing oocytes vitrified before or after IVM indicated that there were no significant differences between examined characteristics.
Sponsor: This research was supported by the University of Alicante VIGROB-186 and UAUSTI14-04, and by the Chair of Human Fertility of the University of Alicante.
URI: http://hdl.handle.net/10045/68851
ISSN: 0916-8818 (Print) | 1348-4400 (Online)
DOI: 10.1262/jrd.2017-009
Language: eng
Type: info:eu-repo/semantics/article
Rights: ©2017 by the Society for Reproduction and Development. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial-No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
Peer Review: si
Publisher version: http://dx.doi.org/10.1262/jrd.2017-009
Appears in Collections:INV - Grupo de Inmunología - Artículos de Revistas

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