Reprint of: l-Cysteine determination in embryo cell culture media using Co (II)-phthalocyanine modified disposable screen-printed electrodes

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Título: Reprint of: l-Cysteine determination in embryo cell culture media using Co (II)-phthalocyanine modified disposable screen-printed electrodes
Autor/es: Hernández Ibáñez, Naiara | Sanjuán, Ignacio | Montiel López, Miguel Ángel | Foster, Christopher W. | Banks, Craig E. | Iniesta Valcárcel, Jesús
Grupo/s de investigación o GITE: Electroquímica Aplicada y Electrocatálisis
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Química Física | Universidad de Alicante. Instituto Universitario de Electroquímica
Palabras clave: Sthiol-containing compounds | l-Cysteine | Cobalt phthalocyanine | Screen-printed electrodes | Embryo cell culture media | Oxidative stress
Área/s de conocimiento: Química Física
Fecha de publicación: 15-may-2017
Editor: Elsevier
Cita bibliográfica: Journal of Electroanalytical Chemistry. 2017, 793: 77-84. doi:10.1016/j.jelechem.2017.04.034
Resumen: Thiol-containing compounds such as l-cysteine have been demonstrated to play an important role in metabolism and cellular growth, acting as powerful antioxidants. Consequently, their analytical determination in biological media has received a considerable amount of attention. In this work, an electrochemical sensor for the accurate electroanalytical determination of l-cysteine is proposed, based upon a Co(II)-phthalocyanine bulk modified disposable screen-printed graphite electrode (CoPc-SPE). Cyclic (CV) and Square Wave (SWV) voltammetry experiments have demonstrated an excellent electrocatalytic activity towards the electrochemical oxidation of l-cysteine using CoPc-SPEs within optimum neutral or basic pH. Moreover, the SWV response of l-cysteine is found to exhibit a linear range of 2.6–200 μM, with a low limit of detection of 4 μM (S/N = 3) and a sensitivity of 0.78 μA cm− 2 μM− 1. Coefficient of variation for reproducibility and repeatability of l-cysteine determination using the CoPc-SPE are 3 and 0.4%, respectively. The effect of inherent interferences such as naturally occurring amino acids, cystine and cysteic acid has been also evaluated. Finally, the applicability of the l-cysteine electrochemical sensor based upon CoPc-SPEs has been successfully demonstrated for the first time for the assessment of l-cysteine in a complex embryo cell culture medium.
Patrocinador/es: J. Iniesta thanks the programme Salvador de Madariaga from the Ministerio de Economía y Competitividad, grant number PRX14/00363. This work has been also financially supported by the MICINN-FEDER (Spain) through the projects CTQ2013-48280-C3-3-R.
URI: http://hdl.handle.net/10045/68569
ISSN: 1572-6657 (Print) | 1873-2569 (Online)
DOI: 10.1016/j.jelechem.2017.04.034
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © 2016 Published by Elsevier B.V.
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1016/j.jelechem.2017.04.034
Aparece en las colecciones:INV - LEQA - Artículos de Revistas

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