Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)

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Título: Morphological, molecular and phylogenetic analyses of the spirurid nematode Stegophorus macronectes (Johnston & Mawson, 1942)
Autor/es: Vidal, V. | Ortiz, J. | Diaz, J.I. | Zafrilla Requena, Basilio | Bonete, María-José | Ruiz De Ybañez, M.R. | Palacios, M.J. | Benzal, J. | Valera, F. | De La Cruz, C. | Motas, M. | Bautista, Vanesa | Machordom, A. | Barbosa, A.
Grupo/s de investigación o GITE: Biotecnología de Extremófilos (BIOTECEXTREM)
Centro, Departamento o Servicio: Universidad de Alicante. Departamento de Agroquímica y Bioquímica
Palabras clave: Spirurid nematode | Stegophorus macronectes | Gastrointestinal parasite
Área/s de conocimiento: Bioquímica y Biología Molecular
Fecha de publicación: mar-2016
Editor: Cambridge University Press
Cita bibliográfica: Journal of Helminthology. 2016, 90(2): 214-222. doi:10.1017/S0022149X15000218
Resumen: Stegophorus macronectes (Johnston & Mawson, 1942) is a gastrointestinal parasite found in Antarctic seabirds. The original description of the species, which was based only on females, is poor and fragmented with some unclear diagnostic characters. This study provides new morphometric and molecular data on this previously poorly described parasite. Nuclear rDNA sequences (18S, 5.8S, 28S and internal transcribed spacer (ITS) regions) were isolated from S. macronectes specimens collected from the chinstrap penguin Pygoscelis antarctica Forster on Deception Island, Antarctica. Using 18S rDNA sequences, phylogenetic analyses (maximum likelihood, maximum parsimony and Bayesian inference) of the order Spirurida were performed to determine the phylogenetic location of this species. Primer pairs of the ITS regions were designed for genus-level identification of specimens, regardless of their cycle, as an alternative to coprological methods. The utility of this molecular method for identification of morphologically altered specimens is also discussed.
Patrocinador/es: This study was funded by the Spanish Ministry of Economy and Competiveness and the European Regional Development Fund Projects CGL2004-01348, POL2006-05175, CGL2007-60369 and CTM2011-24427. V.V. was supported by a PhD grant from the Spanish Council of Scientific Research (CSIC) and the European Social Fund (JAEPre08-01053). M.J.P. was supported by a PhD grant from the Spanish Ministry of Science and Innovation (BES2005-8465). J.I.D. is a member of CONICET and is partially supported by PIP 698 (CONICET), N628 and N726 (UNLP).
URI: http://hdl.handle.net/10045/63432
ISSN: 0022-149X (Print) | 1475-2697 (Online)
DOI: 10.1017/S0022149X15000218
Idioma: eng
Tipo: info:eu-repo/semantics/article
Derechos: © Cambridge University Press 2015
Revisión científica: si
Versión del editor: http://dx.doi.org/10.1017/S0022149X15000218
Aparece en las colecciones:INV - BIOTECEXTREM - Artículos de Revistas

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